小麦Glu-B1位点高分子量麦谷蛋白亚基的功能分析及其调控机理

项目名称： 小麦Glu-B1位点高分子量麦谷蛋白亚基的功能分析及其调控机理

项目编号： No.31271725

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 雷振生

作者单位： 河南省农业科学院

项目金额： 86万元

中文摘要： 小麦面粉中高分子量麦谷蛋白亚基（HMW-GS）的类型和数量直接关系到面筋强度，进而影响小麦的加工品质。研究发现，面筋强度与Glu-B1位点1Bx7超量表达亚基有关，而它的表达增强是由于在含有该亚基的小麦材料中1Bx7增加了1个拷贝。据此，我们推测利用转基因技术在目前生产上大面积推广的品种中增加1Bx7的拷贝数，可以进一步提高小麦品质。本课题采用转基因方法将源于澳大利亚优质小麦材料中的1Bx7亚基导入到郑麦366中，期望获得更加优质的新材料；在国内外首次采用hpRNAi结合amiRNA技术依次分别和同时沉默郑麦366中Glu-B1位点1Bx7+1By8亚基的表达，以分析它们对小麦最终加工品质的影响；拟采用启动子分段缺失、酵母单杂交和凝胶迁移阻滞实验来分离调控Glu-B1位点亚基表达的关键因子。本项目研究结果不仅可以阐明HMW-GS表达的分子机理，还为利用转基技术改良小麦品质提供新思路。

中文关键词： 小麦；高分子量麦谷蛋白亚基；超表达；调控机理；转录组测序

英文摘要： The type and quantity of high molecular weight glutenin subunits (HMW-GS) from wheat flour are directly bound up with the gluten strength, which mainly influences the processing quality of wheat. It has been reported that the gluten strength has something to do with the 1Bx7 overexpression subunit from Glu-B1 locus, and it's the one increased copy of 1Bx7 in the wheat material with 1Bx7 overexpression subunit that cause its overexpression. So we can infer that by increasing the copies of 1Bx7 in varieties which are widely popularized in production using transgene technology, the wheat quality will be further improved. In our research group, the 1Bx7 subunit from high-quality wheat material derived from Australia will be transfered into Zhengmai 366 by transgene method to obtain the higher-quality new material; To analyze the influence of 1Bx7+1By8 subunits on wheat quality, we will be the first at home and abroad to utilize hpRNAi and amiRNA technologies in turn to respectively and simultaneously silence the expression of 1Bx7+1By8 subunits from Glu-B1 locus in Zhengmai 366; We plan to apply promoter stepwise deletion, yeast one-hybrid, and electrophoresis mobility shift assay to identify the key factors which regulate the subunit expression in Glu-B1 locus. The result of this project will not only demonstrate

英文关键词： Bread wheat；High molecular weight glutenin subunit；Overexpression；Regulatory mechanism；Transcriptome sequencing