多肽对纳米颗粒的识别性亲和及其在组装生物功能纳米结构中的应用研究

项目名称： 多肽对纳米颗粒的识别性亲和及其在组装生物功能纳米结构中的应用研究

项目编号： No.21303050

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 王宜冰

作者单位： 华东理工大学

项目金额： 25万元

中文摘要： 功能性纳米结构的构建大多依赖于不同组分间有序和可控的组装与结合。在生物医药领域，许多用于检测和治疗的功能性纳米结构是通过蛋白质等生物分子与纳米颗粒的组装、结合来构建的。其中，抗体与纳米颗粒的组装与结合被广泛用于构建靶向性给药系统。共价连接手段通常用来将抗体与纳米颗粒结合，但得到的纳米结构有抗体取向不一、活性低的弱点。如何有效实现抗体与纳米颗粒的有序组装至今仍是该领域尚未很好解决的重大问题。本项目将研究多肽与纳米颗粒的非共价特异性亲合规律，并通过多肽与抗体的定点偶联，使抗体可借助多肽的亲和作用在纳米颗粒表面定向自组装，实现抗体取向一致的目的，提高抗体与肿瘤细胞表面抗原结合的有效性。同时研究此类抗体-纳米颗粒有序组装结构的稳定性的影响因素，以便实现有序组装结构在血液中的良好稳定性，达到可控药物释放的目的。本项目可望为有序可控组装功能性纳米结构提供新的思路。

中文关键词： 多肽；抗体；酶；纳米结构；自组装

英文摘要： Construction of functional nanostructure relies heavily on oriented and adjustable assemblies of different components. Biomolecules, such as proteins have been widely used as building blocks for functional nanostructures in the design of morden biomedicine. Such nanostructures have been used for detection and therapy purposes. Antibody - conjugated nanoparticles have been explored as drug delivery carriers targeting to tumor cells. Antibodies generally were not well oriented and possess low actives in nanostructures prepared through covalent bonding. How to realize oriented binding of antibodies to nanoparticles still remain as a grand challenge. This study will explore the mechanisms of the affinity interactions between polypeptide and nanoparticle, thus realizing assembling of peptide site - specifically coupled antibody and nanoparticles, forming ordered assemblied systems. It will increase the effectiveness of binding of antibodies to antigens on tumor cell surfaces. This project will also study the impacting factors of stability of such antibody - bound nanostructure, to enable their stabilily in blood and realize controlled drug release. This study is expected to provide a new concept in designing oriented tuneable assemblies for biotechnology applications.

英文关键词： peptide；antibody；enzyme；nanostructure；self-assembly