多波长、荧光数字全息显微术及在T细胞活化定量表征中的研究

项目名称： 多波长、荧光数字全息显微术及在T细胞活化定量表征中的研究

项目编号： No.61275015

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 钟丽云

作者单位： 华南师范大学

项目金额： 82万元

中文摘要： 数字全息显微术是数字全息与光学显微术结合的一种新的测量技术，具有非介入、非干预、非扫描、快速、全场、动态、三维成像和亚纳米量级超高分辨率的独特优点，是物体三维形貌、表面起伏、表面质量、内部折射率检测的有力工具。生理条件下细胞动态行为的定量表征是细胞生物学研究的热点和核心内容之一，而荧光标记是细胞和分子行为探测的重要手段。多波长和荧光数字全息显微术因其量程大和非相干测量特点，在活细胞动态行为和荧光标记细胞及生物大分子的动态行为定量表征方面有很好的应用前景。项目拟在研究解决单波长、双波长及荧光数字全息显微术相位重构和像-物空间反演中存在问题的基础上，建立多波长数字全息和荧光数字全息综合的多功能显微数字全息实验系统，应用该系统对非标记生理条件下的T细胞活化过程中的动态行为，以及荧光标记TCR微域和免疫突触动态行为进行定量表征研究，为研究T细胞活化状态的定量评价和活化信号传导的分子机制提

中文关键词： 数字全息显微术；多波长相移数字全息显微术；荧光数字全息显微术；相位重 构；T细胞

英文摘要： Digital holographic microscopy, which is a kind of dynamic quantitative phase imaging technique with non-invasion, non-intervention, non-scanning, real-time and sub-nanometer resolution, can be used to inspect 3D morphometry and its fluctuation, surface quality of micro-area, the refractive in the measured object. Quantitative characterization the dynamic behavior of living cell in the physiological condition is one of the key research content for cell biology. Fluorescence labeling is a powerful tool to detect the behavior of cell and its molecules. Specially, multi-wavelength digital holographic microscopy with large measuring range, can be used in quantitative characterization the dynamic behavior of living cell in the physiological condition, and fluorescence digital holographic microscopy with non-coherent measurement, can be used in quantitative characterization the cell and its molecules labeled by fluorescence probe . In this project, firstly, the phase reconstruction algorithm, the image-object space inversion for single-wavelength, multi-wavelength and fluorescence digital holographic microscopy will be studied carefully, and then the multi-functional integrated system of multi-wavelength digital holographic microscopy and fluorescence digital holographic microscopy with will be set up. Finally, this i

英文关键词： Digital holographic microscopy；Multi-wavelength digital holographic microscopy；fluorescence digital holographic microscopy；Phase reconstruction；T cell