脊髓损伤早期治疗中磁纳米膜修复材料的靶向定位研究

项目名称： 脊髓损伤早期治疗中磁纳米膜修复材料的靶向定位研究

项目编号： No.51307166

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 电工技术

项目作者： 张广浩

作者单位： 中国科学院电工研究所

项目金额： 26万元

中文摘要： 脊髓损伤后及时且有效的早期修复可以极大改善患者的预后。前期实验表明直流电刺激补偿损伤电位可有效阻止损伤后阳离子内流，维持细胞膜内外离子平衡，从而延长有效治疗时间窗口。本项目拟在该时间窗口内利用磁纳米膜修复材料将受损伤的细胞膜缝合,并通过构建靶向定位系统，解决损伤处膜修复材料滞留率低的问题，更好的抑制继发性损伤。离体实验中利用诱发电位研究膜修复材料浓度与损伤修复程度的关系，找到修复效果最好时的损伤处最佳浓度。动物实验中在体检测损伤处膜修复材料浓度，验证靶向定位效果，确定可达到损伤处最佳浓度的注射剂量与时间；采用损伤电位补偿结合磁纳米膜修复材料靶向定位的方法治疗实验动物脊髓损伤，评价其远期治疗效果及磁纳米膜修复材料的毒性。最终通过本项目研究为用于脊髓损伤早期治疗的磁纳米膜修复材料及其靶向定位系统的未来临床应用提供理论基础。

中文关键词： 磁靶向；脊髓损伤；膜修复；；

英文摘要： Successful membrane resealing after spinal cord trauma without delay can significantly improve the prognosis. Preliminary study has shown that compensation for injury potential by direct current stimulation can effectively prevent influx of cations and maintain the ionic balance between intra- and extracellular space and prolong the effective time of treatment, in which the injured membrane is assumed to be resealed by magnetic membrane repair nanomaterial in this project. In order to improve the retention rate of membrane repair material at injury site and thus inhibit the secondary injury more effectively, a site directed magnetic membrane repair nanomaterial targeting system will also be constructed. The relationship between concentration of magnetic membrane repair nanomaterial and effect of repair will be investigated in vitro, with the aim of seeking out the optimal concentration at the injury site. In animal experiment, the concentration of magnetic membrane repair nanomaterial at injury site will be detected to evaluate the effect of the site-directed system and to seek out the dose and time used to achieve the optimal concentration. Then compensation for injury potential and site-directed magnetic membrane repair nanomaterial targeting will be combined in animal experiment to treat acute spinal cord tr

英文关键词： magnetic targeting；spinal cord injury；membrane resealing；；