钙信号系统调控香蕉耐盐生理和分子机制研究

项目名称： 钙信号系统调控香蕉耐盐生理和分子机制研究

项目编号： No.31260462

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 李新国

作者单位： 海南大学

项目金额： 45万元

中文摘要： 香蕉是我国具有国际竞争力的优势农产品之一，但是生产中盐碱化和干旱严重限制其产业的发展。钙作为重要的的第二信使，参与许多植物对逆境信号的传导。本项目紧密结合我国香蕉产业存在的问题，拟采用耐盐性不同的香蕉品种，在不同种类、浓度和作用时间的人工模拟盐胁迫逆境下，利用外源Ca2+、TFP、A23187等试剂设置阻碍或增强钙信号转导途径的处理，分别从植株整体、细胞、及分子等各种响应层面上，研究盐胁迫前后香蕉的Ca2+超微细胞化学定位、CaM含量和Ca2+-ATPas活性及其基因表达、盐诱导的特异蛋白质时空表达、以及其形态结构、生长发育、生理生化等耐盐指标变化，探析钙信号系统调控香蕉耐盐生理与分子机理。本项目的完成将在理论上丰富香蕉耐盐调控的解析机理；在实践上指导香蕉的耐盐育种和耐盐优质栽培打下基础，促使香蕉产业可持续发展。

中文关键词： 香蕉；盐胁迫；钙信号；生理机制；分子机制

英文摘要： Banana is an important agricultural product of international competitiveness. But soil salinization and drought injury are two most important factors limiting the development of banana industry. Calcium acts as a secondary messenger and plays a critical role of stree signaling transdution in many plants. Base on above questions on banana industry of China, banana plantlet with different salt tolerance are investigated under saline stress with different species, contentrations and treatment time by the methods of pot. Then some chemical reagents which are including extragenous Ca2+,calmodulin antagonist TFP or Ca2+ transport A23187 and so on, are added during different salt injury stage. From theree levels of the whole plant, celluar and molecular response, we study ultracytochemical localization of calcium, change on calmodulin content and Ca2+-ATPase activitise and these gene experssion, temporal and spatial expression patterns of indcued especial protein, changes of morphological traits, growth and development, physiological and biochemical indexes regulted by calcim singnaling system. The aims of the research are to apply the base of breeding new cultilvars with saline resistence, take availble practical measures to prevent saline injury, and promote sustainable development for banana industry.

英文关键词： banana；salt-stress；calcium signal；physiological mechanism；molecular mechanism