雌激素降解工程菌的构建与代谢调控机理研究

项目名称： 雌激素降解工程菌的构建与代谢调控机理研究

项目编号： No.51478096

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 其他

项目作者： 霍洪亮

作者单位： 东北师范大学

项目金额： 90万元

中文摘要： 雌激素是一种典型的环境内分泌干扰物，受多环杂化形成的空间位阻效应影响,难以被微生物降解，在许多城市污水处理厂的外排水中都能检测到其存在。当前，针对水中雌激素生物降解的研究，多集中在工艺条件优化和专性降解菌特性分析层面，仅有少数报告涉及基因增强技术应用于去除水中难降解有机物。本项目以典型雌激素--雌二醇、双酚A和壬基酚为研究对象，开展基因修饰强化生物降解水中雌激素的研究，重点体现在以下三个方面：（1）解析专性降解菌的特异基因片段，阐明菌株对典型雌激素的代谢机制；（2）基因重组拓宽菌株底物利用范围，提高对雌激素的降解效率；（3）分析重组菌的特异基因表达，获得重组菌生物强化活性污泥法的调控方法。通过上述研究，可以从基因水平上强化专性菌株的底物利用范围和降解能力，获得基因工程菌应用于生物强化活性污泥法的关键控制方法，为基因技术应用于污水生物处理系统提供理论指导与技术支持。

中文关键词： 污水处理；内分泌干扰物；生物强化；基因表达；工程菌

英文摘要： Estrogen, a kind of typical endocrine disruptors (EDCs), increasingly exists in urban water system. As estrogen, even at low concentration level, can contribute to animal endocrine disorder and feminization, and due to its characteristics of persistence and hard biodegradation, estrogen seriously threatens urban water quality and ecological security. This project takes the typical estrogen-estradiol, bisphenol A and nonylphenol as the research object, study the gene enhanced biological degradation of estrogen, mainly reflected in the following three aspects: analysis of bacterial degradation specific gene positioning, speculated that the metabolic substrate strain; Gene chip analysis and expression mechanism research of keymetabolic genes of estrogen Degradation Strains; gene recombination to broaden the scope of the substrate utilization, improve the environmental estrogen removal efficiency; the comprehensive utilization of gene chip, RT-PCR technology analysis of recombinant strains for biological enhancement system, law and community specific gene expression in activated sludge evolution characteristics. Through the above study, metabolic capacity from gene level to strengthen the traditional specific strains, and improve the activity of sludge removal efficiency of water of estrogen, gene technology applied to strengthen the biological wastewater treatment system, to provide theoretical guidance and technical support.

英文关键词： wastewater treatment;endocrine disrupter;bioaugmentation;gene expression;bioengineered strain