基于生物可利用机制的沉积物中拟雄激素活性物质鉴别研究

项目名称： 基于生物可利用机制的沉积物中拟雄激素活性物质鉴别研究

项目编号： No.21307054

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 环境科学、安全科学

项目作者： 史薇

作者单位： 南京大学

项目金额： 26万元

中文摘要： 拟雄激素活性物质广泛存在于各种环境介质特别是沉积物中，导致了雌鱼雄性化等生态问题的产生，然而目前缺乏拟雄激素活性物质的系统研究，关键毒物结构和种类不明确。围绕如何真实萃取沉积物中可生物利用的拟雄激素活性物质，并进行毒物鉴别的技术需求，本项目拟利用助解析材料，研究代表性拟雄激素活性物质在沉积物中的解析释放过程，揭示其对生物可利用性的影响；在此基础上，针对表皮交换作用与摄食作用，研究筛选符合生物可利用机制的仿生萃取材料，建立仿生萃取技术，以模拟真实暴露情况；并进一步通过分级分离、受体报告基因测试、目标与非目标物鉴别相联合的手段，建立起适用于沉积物拟雄激素活性物质鉴别的技术方法。在前期研究的基础上，进一步将该技术应用于太湖北部湖区，鉴别沉积物中的关键拟雄激素活性物质，阐明其污染特征及分布差异，为区域环境雄激素污染控制提供技术支撑。

中文关键词： 拟雄激素活性；沉积物；目标识别；非目标识别；风险评价

英文摘要： Androgenic compounds are widespread in the environment especially in sediments. They have posed great threat to the aquatic systems including fish virilization. However,little in-deep research has focused on the primary toxicants identification. It is quite necessary to extract the bioavailable androgenic compounds in sediments and further identify the responsible compounds. The three objectives of this study are to: 1) Study the desorption process of androgenic compounds from sediments. Build analytic dynamics model to reveal the bioavailability of androgenic compounds. Choose available bionic-extraction materials that follows the bioavailability mechanisms based on incomplete extraction and in vitro digestive fluid extraction. Establish the methods of bionic-extraction to simulate real exposure conditions. 2) Establish practical methods for identification of toxicants in sediments by integrating fractionations, toxicity testing and qualitative analysis. 3) Based on the previous study, the established methods will be further used for the identification of androgenic compounds in northern Taihu Lake. Pollution characteristics and spatial distribution of the responsible androgenic compounds in this region will be elucidated. This study will provide decision-making support for the pollution control of environmenta

英文关键词： Androgenic activity；Sediment；Target analysis；Non-target analysis；Hazard assessment