纳米羟基磷灰石/聚醚醚酮对成骨细胞生物效应的定量蛋白质组学研究

项目名称： 纳米羟基磷灰石/聚醚醚酮对成骨细胞生物效应的定量蛋白质组学研究

项目编号： No.81500900

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 赵敏芝

作者单位： 国家纳米科学中心

项目金额： 18万元

中文摘要： 热塑性聚合物材料聚醚醚酮具有优越的理化性能，是较受瞩目的骨植入材料，但其需要与羟基磷灰石等生物活性材料复合以改变其生物惰性。研究表明纳米化的羟基磷灰石生物活性更佳，而纳米材料用于人体内的安全性问题备受关注，用其制备的复合材料的最终效应更为重要。现有医用材料标准检测体系中的方法大多停留在细胞水平，不能满足全面深入了解材料与机体相互作用的要求。蛋白质组学技术的发展使生命科学从对单个蛋白孤立研究发展到系统性研究的崭新阶段，为纳米植入材料的全局生物效应评价和分子机理研究提供了有效途径。本课题关注纳米羟基磷灰石与聚醚醚酮复合后对其生物相容性的影响。运用定量蛋白组学技术，研究成骨细胞响应复合材料作用所呈现的蛋白质表达规律、特征蛋白网络及关键信号通路，从分子水平整体层面理解不同“惰性”基体材料生物效应的差异及共性，进一步探讨聚醚醚酮中所含高浓度纳米羟基磷灰石在其生物效应中发挥的作用。

中文关键词： 纳米羟基磷灰石/聚醚醚酮；生物材料；定量蛋白质组学；生物相容性；纳米生物效应与安全性

英文摘要： Thermoplastic polymer material polyetheretherketone which has superior physical, chemical and mechanical properties, is a widely anticipated bone implant material. However it usually needs to composite with other materials to improve its bio-inert performance. Hydroxyapatite is regarded as an active material, which is commonly used as composite additives, and the excellent biological properties of nano-hydroxyapatite has drawn more attention. Meanwhile in the field of biomedicine application, greater emphasis is also being placed on the security problem of nanomaterials. The existing medical material safety evaluation methods mostly stays at a cellular level and cannot satisfy the requirements of insight into the interaction between material and the body. The appearance of” –mics” technology make life sciences develop from the single molecule research to a new stage of the high throughput research. It provide an effective way for the study of the integral biological effects and molecular mechanism of the nano-materials and implant materials. By employing quantitative proteomic techniques, this project focus on the comprehensive biocompatibility of nanometer hydroxyapatite composited with polyetheretherketone which contacts directly with osteoblast, to obtain the whole proteins expression regularity and the key signaling pathways, understand the generality and difference of different “bio-inert” substrate materials and to analyze the role of high concentrations of nano-hydroxyapatite mixed with the inert substrate materials in the biological effect from the global molecular level.

英文关键词： nano-hydroxyapatite/polyetheretherketon(n-HA/PEEK);Biomaterial;Quantitative Proteomics;Biocompatibility;Biological Effects and safty of Nanomaterial