海水营养盐微流控芯片分析平台的构建及其应用基础研究

项目名称： 海水营养盐微流控芯片分析平台的构建及其应用基础研究

项目编号： No.41206076

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 海洋科学

项目作者： 曹煊

作者单位： 山东省科学院

项目金额： 25万元

中文摘要： 被《Nature》杂志誉为"这一世纪的技术"的微流控芯片技术已在生物、医药和化学等领域显示出强大的生命力，然而这项技术在海洋科学尤其是海洋化学领域的应用才刚刚起步，尚有许多基础性的问题有待研究。 本课题选取海水营养盐这一海洋化学中基础却很重要的参数为研究对象，以探索微流控芯片的海洋适用性为目标，构建基于微流控芯片的海水营养盐分析平台。围绕该芯片平台的可靠性、稳定性和灵敏度三个因素展开基础性研究，包括建全特氟龙PFA材料芯片以提高分析系统的抗腐蚀性、抗生物附着性以及降低试剂吸附性，构建混合和反应动力学模型以筛选最佳微混合形式，开发和优化微透镜和光屏蔽技术以提高检测系统的灵敏度和稳定性等。本课题的开展为开发高集成度、低成本的现场（或原位）海水营养盐传感器提供一个新的研究思路，同时也为微流控芯片技术更快更好的应用于海洋研究领域提供理论和实验的依据。

中文关键词： 微流控芯片；海水营养盐；海洋监测；原位传感器；PMMA芯片

英文摘要： Microfluidic chip， which was honored as "the technology for this century" in NATURE, had shown its great vitality in the field of biology, medicine and chemistry in recently years. However, this technology is in its infancy in marine science, and a lot of basic problems are in suspense. In this subject, marine nutrients, very basic but important parameters in marine chemistry, are chosen as the research object. A new seawater nutrient analysis platform based on microfluidic chip is developed to investigate the survivability of microfluidic chip in marine environment. Research of reliability, stability and sensitivity on platform are detailedly depolyed, including the development of whole-Teflon chip to decease the corrosivity, biological adhesion and adsorption of reagents in the microchannels, establishment of mixing and reaction kinetics models to optimize the pattern of the micromixing part,and, application of microlens and light screening technology to enhance the sensitivity. This subject provide a new protocol to develop high integrated level, low cost on situ (in situ) seawater nutrient sensor.Moreover, with the proceeding of this subject and breakthrough achievement, The results of this subject would give theoretical and experimental supports to apply microfluidic chip in the field of marine science.

英文关键词： Microfluidic Chip;；Seawater Nutrients；Marine Monitoring；In-situ sensor；PMMA chip