血清长链非编码RNA- - 环境化学致癌新生物标志物及其功能探索

项目名称： 血清长链非编码RNA- - 环境化学致癌新生物标志物及其功能探索

项目编号： No.21277036

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 环境科学、安全科学

项目作者： 蒋义国

作者单位： 广州医科大学

项目金额： 80万元

中文摘要： 环境化学物是肺癌重要病因，近年非编码RNA尤其小分子RNA与肿瘤关系倍受关注。本课题组化学致癌小分子RNA研究已在国际毒理学权威杂志发表多篇论文，由于长链非编码RNA(lncRNA)蕴含更丰富信息更具意义，已将研究拓展到lncRNA，细胞实验已明确了lncRNA在癌变中的作用。我们开创性地启动了血液lncRNA探索，前期实验检测到血清lncRNA存在，初步发现肺癌动物血lncRNA异常。本项目拟以苯并(a)芘诱发小鼠肺癌，鉴定癌发不同时期血清lncRNA表达，分析血与肺lncRNA表达关联性；在体外细胞实验中筛选有功能意义lncRNA，最终在小鼠体内鉴定血液lncRNA在化学致癌中作用。本研究将探寻出肺癌动物血中lncRNA标志物，并确定其功能，不仅为肺癌诊断提供非创伤检测新标志物，更为将来人群中开展有害暴露检测提供了新方法，还可对其他环境疾病的血液lncRNA研究起到示范效应。

中文关键词： 化学致癌；生物标志物；长链非编码RNA；血液；

英文摘要： Environmental chemicals are considerable cause of lung cancer. Noncoding RNAs, especially small molecule RNAs, are increasingly recognized to play major regulatory role in carcinogenesis in recent years. Our group has published some papers about small molecule RNAs involved in chemical carcinogenesis in international authoritative toxicological journals. In considerate that the long noncoding RNAs (lncRNA) contain much more information and thus more meaningful, we have expanded our research to lncRNA and have found the role of lncRNAs in carcinogenesis in cell experiments. We initiated an innovative exploration in blood lncRNA. We have identified the existence of serum lncRNA and discovered the abnormal expression of blood lncRNAs in lung cancer animals in a preliminary experiment. In this new project, we intend to establish a benzo(a)pyrene-induced lung cancer mice model, identify the expression of serum lncRNAs in different periods of carcinogenesis and analysis the correlation between lung lncRNA expression and blood lncRNA expression. We will discover the functional lncRNAs with in vitro cell experiments, and ultimately appraise the role of blood lncRNAs in chemical carcinogenesis by in vivo animal studies. This project will investigate the blood lncRNAs as lung cancer biomarker and understand their function

英文关键词： chemical carcinogenesis；biomarker；long noncoding RNA；blood；