婴儿CMV肝炎的病证代谢组学研究

项目名称： 婴儿CMV肝炎的病证代谢组学研究

项目编号： No.81473725

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 杨燕

作者单位： 首都医科大学

项目金额： 74万元

中文摘要： 本项目组通过大量婴儿CMV肝炎中医药有效性的临床验证和中药有效方剂的基础研究，不断发展、完善了中医药对婴儿CMV肝炎的辨证论治体系，制定了《中医儿科常见病诊疗指南o胎黄》。前期研究发现，CMV肝炎患儿不同证候分类存在各自的证候特点和生物学特性，而代谢组学方法可在中医证候宏观辨证的基础上，阐释机体的内在微观变化，即发挥微观辨证的优势。为进一步研究婴儿CMV肝炎不同中医证候与代谢组学的关联性，本项目组拟采用液相色谱-质谱（LC-MS）联用的技术方法，通过对正常婴儿各项生物标记物检测的对照分析，研究CMV肝炎不同证候患儿间、与正常婴儿间血清、尿液的代谢组学特征、规律及表达差异，全面分析其特征性代谢模式，发现特征性代谢物谱，建立证相关指纹代谢特征图谱库，进一步探讨婴儿CMV肝炎的发病机制。

中文关键词： 婴儿；CMV肝炎；中医证候；代谢组学

英文摘要： Cytomegalovirus (CMV) hepatitis is the most common during the neonatal and infant periods. In our previous studies, Chinese medical syndrome differentiation of infantile CMV hepatitis has been established by a lot of clinical and basic research. The study shown that the presence of CMV hepatitis with different syndromes has their different biological characteristics; such biological characteristics affected the clinical outcome to a certain extent. And metabonomics based on TCM syndrome differentiation of macroscopic, can interpret the body's internal microscopic changes, which is to play the advantages of microcosmic syndrome differentiation. For the further study of the relevance about infantile CMV hepatitis in TCM syndromes and metabonomics, the project intends to adopt the technology of liquid chromatography - mass spectrometry (LC - MS) combination, studies of different syndromes CMV hepatitis serum, urine metabolomics features, regular and express differences, roundly analyzes its characteristic metabolic pattern, finds characteristic metabolic spectrum, establish the related metabolic fingerprint feature atlas database, discusses the pathogenesis, through the contrast analysis for the various biomarkers detection of the normal infants.

英文关键词： infants;CMV hepatitis;syndrome;metabolomics