泰山天花中抗细菌性咽炎物质的分离鉴定及机理初探

项目名称： 泰山天花中抗细菌性咽炎物质的分离鉴定及机理初探

项目编号： No.31501813

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 朱孟娟

作者单位： 山东农业大学

项目金额： 20万元

中文摘要： 咽炎是耳鼻喉科常见的一种疾病，也是最常见的呼吸道感染病症，具有顽固性、多发性和难治愈等特点，令耳鼻咽喉科医师感到棘手，也影响人们的身体健康和正常生活，而泰山民间素有“泰山天花治疗咽炎”的历史。本研究以此为切入点，利用本团队分离纯化活性物质的技术优势，以咽炎致病菌为指示菌，对泰山天花中治疗咽炎的活性物质进行分离纯化并最终鉴定，确定泰山天花中具体何种物质在起作用，然后利用细菌性咽炎动物模型检测分离纯化出的活性物质对咽炎的治疗效果，并结合体征观察、组织病理学观察、细胞因子及炎症因子检测、蛋白质组学分析等方法，初步分析此活性物质对动物细菌性咽炎的作用机理。本研究通过对泰山天花治疗咽炎这一民间处方的研究，揭示此民间处方的科学真相，并为这一咽炎治疗方法的推广提供科学依据，具有重要的研究价值。同时，从泰山天花中提取出的活性物质还可以作为治疗咽炎的潜在药物，为咽炎的药物治疗提供材料来源。

中文关键词： 泰山天花；细菌性咽炎；活性物质；分离鉴定；机理

英文摘要： Pharyngitis is a common disease of otolaryngology and respiratory infection which becomes a difficult problem for doctors to resolve because of its stubborn, multiple and difficult to cure. The pharyngitis seriously affects the health of humans, but there is a history of “Grifola frondosa (Taishan) curing pharyngitis” in Taishan. So we take it as the breakthrough point and use our technological advantage of isolation and purification of active substance to isolate and purify the active material from Grifola frondosa (Taishan) with the pathogenic bacteria of pharyngitis as indicator. Then, the active substance will be identified and studied the therapeutic effect with animal model of bacterial pharyngitis. Besides, the specific role of the active substance in the treatment of pharyngitis will be preliminarily studied using vital signs observation, histopathological observation, cytokines and inflammatory cytokines detection and proteomic methods. This research scientifically study and reveal the truth of the folk prescription of “Grifola frondosa (Taishan) curing pharyngitis” and provide the scientific basis for its extension which has important research value. At the same time, the active material purified from Grifola frondosa (Taishan) can be served as the potential drugs for treating pharyngitis and becomes the source of drug treatment of pharyngitis.

英文关键词： Grifola frondosa (Taishan);bacterial pharyngitis;active substance;isolation and identification;mechanism