对虾白斑综合症病毒（WSSV）主要囊膜蛋白相互作用位点的鉴定

项目名称： 对虾白斑综合症病毒（WSSV）主要囊膜蛋白相互作用位点的鉴定

项目编号： No.31272698

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 杨丰

作者单位： 国家海洋局第三海洋研究所

项目金额： 80万元

中文摘要： 对虾白斑综合症病毒（white spot syndrome virus，WSSV）是一种危害全球对虾养殖业的最主要病原。自1993年该病毒爆发以来，造成了巨大的经济损失，但至今尚无有效的控制技术。尽管目前已有证据表明病毒4种与病毒感染和装配密切相关的主要囊膜蛋白VP28、VP26、VP24和VP19之间存在着广泛的相互作用，但至今仍未揭示它们之间相互作用的精确结合区。通常病毒蛋白质之间相互作用的结合位点是研究抗病毒药物的重要基础，因为这些位点有可能是抗病毒药物作用的重要环节与靶点，这也是阻断剂药物开发的基本策略。本申请项目拟在课题组已取得的工作基础上，进一步开展WSSV主要囊膜蛋白之间相互作用结合位点的研究，通过精确定位结合区，分析其氨基酸序列，合成特异性小肽，评估这些肽对囊膜蛋白互作的干扰或阻断能力，为今后开发针对性的靶向药物提供可能的作用靶标，为预防与治疗WSSV奠定分子基础。

中文关键词： WSSV；囊膜蛋白；相互作用；结合位点；

英文摘要： White spot syndrome virus (WSSV) is one major pathogen in global shrimp aquaculture. Since its first outbreak in 1993, the disease has caused huge economic losses, but so far, no effective prevention or therapy strategies are available. Although there is considerable evidence demonstrating extensive interactions between the four major viral envelope proteins VP28, VP26, VP24 and VP19 and that they play pivotal roles in virus infection and assembly, the precise binding regions of interaction between them are not understood. Generally, the interaction binding sites between the viral proteins serve as important targets for potential antiviral drug design to develop target-specific inhibitors, which is essential for many drug based therapeutic strategies. This application builds on our previous work. Therefore, the major goal of the project is to examine the precise binding regions between these viral main envelope proteins. Subsequently, the amino acid sequence of these binding sites will be used to design synthetic peptides and to evaluate whether these peptides can interfere with the interactions between major viral envelope proteins. This project will help to discover potential therapeutic targets or methods for prevention and treatment of this disease.

英文关键词： WSSV；envelope protein；interaction；binding site；