稻米中铅的化学形态及其细胞毒性机理研究

项目名称： 稻米中铅的化学形态及其细胞毒性机理研究

项目编号： No.31471680

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 方勇

作者单位： 南京财经大学

项目金额： 88万元

中文摘要： 近年来，我国稻米铅含量超标事件常有发生，在食品安全领域逐渐成为强烈关注的对象。目前食品安全标准以总铅为限量指标，并未对铅的形态做明确规定。然而，各种铅的化合物毒性不同，并且在细胞内有效性和毒性机理各不相同，铅的化学形态与稻米食用安全存在密切联系。针对这一尚待研究解决的问题，本项目拟在前期研究基础上，（1)采用金属组学手段，分析高铅稻米中铅的主要形态；（2）建立3种体外细胞模型（小鼠巨噬细胞、大鼠神经细胞和成骨细胞），系统地评价稻米中主要铅化合物的细胞毒性及作用剂量范围；（3)利用体外消化/Caco-2细胞模型，明确稻米中铅的化学形态与生物有效性相互关系，并进一步分析3种染毒细胞关键蛋白酶、细胞因子和基因转录水平，从分子与细胞水平揭示铅的化学形态与细胞靶向毒性作用机制。通过铅的质与量两方面评价其形态与毒性的关系，为合理的表征稻米中铅的真实毒性及其食用安全性提供科学依据。

中文关键词： 稻米；铅；化学形态；细胞毒性；食品安全

英文摘要： In recent years, the exceeding limitation of lead (Pb) was occasionally found in Chinese rice, which gradually become the objective to the researchers in the food safety area. So far, the total Pb content has been targeted as a limitation index of rice in national food safety standards, while the Pb speciation is not clearly definite. However, the bioavailability and cytotoxicity mechanism of each Pb compound are individually different. Therefore, the has a close connection with the edible safety of rice. In order to solve the question, our project, with the results of previous studies, will focus on: (1) The analysis of Pb speciation in high Pb content rice by metallomics methods; (2) the evaluation of the cytotoxcity and concentrations of major Pb compounds in rice by the establishment of three cell models (mice macrophage, rats nerve cell and osteoblast); (3) the clarification of the connection between Pb speciation and it bioavailability by in vitro digestion/Caco-2 cell model, and the reveal of the targeted cytotoxic mechanism of Pb compounds via molecular and cell level by further analysis of key proteinese, cell cytokines and gene transcription level of three cells. With the understanding of the relation between Pb speciation and toxicity by the quality and the quantity of Pb, we will build a scientific basis for the reasonable measure of the real toxicity of Pb in rice and its edible safety.

英文关键词： Rice;Lead;Chemical Speciation;Cytotoxicity;Food Safety