动态高压微射流对黄酮类化合物的提取和抗氧化活性的影响机理研究

项目名称： 动态高压微射流对黄酮类化合物的提取和抗氧化活性的影响机理研究

项目编号： No.21276118

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 化学工业

项目作者： 涂宗财

作者单位： 江西师范大学

项目金额： 80万元

中文摘要： 前期研究发现，动态高压微射流（DHPM）辅助提取黄酮可提高其提取率和抗氧化活性，但提取过程中DHPM的强化传质机理，DHPM助提对黄酮抗氧化活性的影响机理还不清楚。本项目针对上述科学问题，在前期研究的基础上，以甘薯叶黄酮(SPLF)为研究对象，通过分析提取条件对提取率的影响及甘薯叶细胞结构来研究其提取行为，并计算传质速率，采用数学拟合结合Fick扩散定律建立DHPM辅助提取SPLF的传质动力学方程；采用紫外光谱、红外光谱、核磁共振和多维色谱-质谱联用技术表征不同提取条件下SPLF的组分；建立四种抗氧化模型研究不同提取条件下SPLF抗氧化活性的差异，并借助统计学构建SPLF抗氧化活性与其组分之间的关系，从而探明其强化传质机理，探清DHPM助提对SPLF抗氧化活性的影响机理，从而为将动态高压提取技术发展为具有潜力和竞争力的新技术提供理论依据，为高效制备高活性天然黄酮类化合物提供理论指导。

中文关键词： 动态高压微射流；多酚；提取；抗氧化活性；机制

英文摘要： Previous research showed that the dynamic high pressure microfluidization(DHPM)-assisted extraction could enhance the extraction efficiency and antioxidant activities of flavonoid , but the enhancing mass transfer mechanism during the DHPM-assisted extraction process and the mechanism of DHPM-assisted extraction influencing the antioxidant activities of flavonoid were still unknown. Aiming at the those scientific issues and based on the former study, sweet potato leaves flavnoid(SPLF) was selected as the research subject, the extraction behaviour was studied by analyzing the influence of extraction condition on the SPLF yield and by observing the cell structure of sweet potato leaves, then the mass transfer rate was calculated, and the mass transfer kinetic equation of DHPM-assisted extraction of SPLF was built by mathematical simulation combined with Fick diffusion law. The UV-Vis spectrom, FT-IR, NMR and chromatograph-MS technology were adopted to characterize the constituents of SPLF extracted under various conditions. The diversity of antioxidant activities of SPLF extracted under different conditions was studied by four antioxidant models. Combined with the relations between antioxidant activities and constituents built by statistical method, the mechanism of DHPM-assisted extraction affecting the antioxid

英文关键词： Dynamic high pressure microfluidization；Polyphenols；Extraction；Antioxidant activities；Mechanism