整合MRI药代动力学灌注参数与ADC值评估乳腺癌新辅助化疗残留病灶及乏氧与组织间隙液压微环境

项目名称： 整合MRI药代动力学灌注参数与ADC值评估乳腺癌新辅助化疗残留病灶及乏氧与组织间隙液压微环境

项目编号： No.81501435

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 刘莉

作者单位： 复旦大学

项目金额： 18万元

中文摘要： 乳腺癌新辅助化疗后残留病灶及肿瘤乏氧、组织间隙液压对治疗方式的选择有重要意义，但目前缺乏准确且无创的评估方法。我们前期研究表明MRI药代动力学灌注与ADC值能够准确评估乳腺癌新辅助化疗疗效及反映肿瘤内部的环境，因此拟使用MRI药代动力学灌注与ADC图融合技术来提高乳腺癌新辅助化疗残留病灶诊断的准确性及评估肿瘤乏氧、组织间隙液压。首先，使用该融合技术评估新辅助化疗后残留病灶，与病理学对照，评估诊断残留病灶数量及范围的特异度；其次，联合MRI药代动力学灌注参数与ADC值评估乳腺癌的乏氧标志物（HIF－1α、GLU-1），建立定量的评价标准；最后，使用该融合技术评价乳腺癌裸鼠的组织间隙液压，并研究组织间隙液压及灌注参数对ADC值的影响。预期研究结果将建立整合MRI药代动力学灌注与ADC值诊断乳腺癌新辅助化疗残留病灶的定量标准，可以成为评估肿瘤微环境的手段，进一步阐明ADC值的形成机制。

中文关键词： 磁共振成像；乳腺癌；残留病灶；组织间隙液压；乏氧

英文摘要： Studies have provided substantial evidence that residual disease in patients receiving neoadjuvant chemotherapy(NAC) ,hypoxia and interstitial fluid pressure (IFP) is critical for planning treatment.To date it is hard noninvasive evaluated accurately residual disease in patients receiving NAC, hypoxia and IFP in breast cancer. In earlier work,We have proved that pharmacokinetic DCE-MRI and ADC value can evaluate accurately treatment response in patients receiving NAC,and referred to environment of tumor.We propose integration of pharmacokinetic DCE-MRI and ADC mapping that is able to noninvasive evaluate accurately residual disease in patients receiving NAC,and assess hypoxia and IFP of breast tumor.Firstly, to use the integration of mapping for the assessment of residual tumor extent and quantity in breast cancer patients receiving NAC,and compared between the radiologic and histopathologic result. To evaluate the accuracy of integration of mapping for the assessment of residualtumor extent in breast cancer patients receivin NAC.Secondly,to study the relationship of DCE-MRI parameters and ADC values with HIF-1α and GLUT－1,and set up quantification assessement criterion. Finally, the integration of mapping and measurement of IFP will be carried out with breast cancer-bearing mice,it is to study the relationship of DCE -MRI parameters and ADC values with IFP ,and investigate whether DCE -MRI parameters and IFP may affect ADC value. Our expected results is that set up quantification assessement criterion in DCE-MRI parameters and ADC values evaluation of residual residual disease in patients receiving NAC,DCE-MRI parameters and ADC values can be expected to be a useful method for assessing hypoxia and IFP of meningiomas, for may be helpful in unraveling mechanism of ADC values.

英文关键词： magnetic resonance imaging;breast neoplasms;residual disease;interstitial fluid pressure;hypoxia