五子衍宗丸调控精子线粒体通透性转换孔（mPTP）治疗少弱精子症机制研究

项目名称： 五子衍宗丸调控精子线粒体通透性转换孔（mPTP）治疗少弱精子症机制研究

项目编号： No.81473674

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 王桐生

作者单位： 安徽中医药大学

项目金额： 73万元

中文摘要： 少弱精子症是常见疑难病症，其发病率呈上升趋势，目前现代医学无特效治疗手段。五子衍宗丸为治疗该症的经典补肾方药，我们前期研究发现，五子衍宗丸通过保护精子线粒体发挥治疗作用。而线粒体通透性转换孔(mPTP)的开放程度直接影响线粒体的结构与功能，故推测mPTP为五子衍宗丸治疗少弱精子症的调控靶点之一。本课题以mPTP为切入点，分别采用苍术苷和雷公藤多苷制备mPTP开放和少弱精子症模型，以mPTP调控因素为研究核心，观察五子衍宗丸对精子电压依赖性阴离子通道（VDAC）表达、腺嘌呤核苷酸转位酶（ANT）含量的影响，以及对Bcl-2家族表达、mPTP开放程度、线粒体膜电位水平、超微结构的干预作用，分析其调控机制。该研究考察mPTP与少弱精子症的内在联系和五子衍宗丸的调控机制，将可能为揭示肾虚精亏的分子机制、寻找新型有效的治疗途径提供崭新思路，为阐述补肾法的现代科学内涵提供新视角。

中文关键词： 五子衍宗丸；少弱精子症；线粒体通透性转换孔；分子机制

英文摘要： Oligoasthenospermia is a common incurable diseases of the male reproductive system，the incidence rate shows an upward trend,previous studies found that Wu Zi Yan Zong pill could tream this disease by protecting sperm mitochondria.While the openness of the mitochondrial permeability transition pore (mPTP) directly affects the structure and function of mitochondria and determines sperm survival, therefore we presume that mPTP might the regulatory targets of wuziyanzong pill on oligoasthenospermia. In this topics the wuziyanzong pill is to be the object and the mPTP as a starting point.The herb Tripterygium glycosides and glycoside preparation were used respectively to make mPTP open cell model and oligoasthenospermia rats, the sperm quality,mPTP opening,mitochondrial enzyme activity,membrane potential(MMP), ultrastructure were observed according to the openness of sperm mPTP, the effects of wuziyanzong pill on the expression of voltage-dependent ion channel (VDAC) ,the content of adenine nucleotide translocation of the enzyme (ANT), the expression of Bcl-2 family were studied by westblot, RT-qPCR, immunohistochemistry methods, and analyzed the mechanism of mPTP regulation. The study examined the internal relations of mPTP and oligoasthenospermia and researched the mechansim of Wu Zi Yan Zong pill regulation. According to the topics,we will likely to reveal the molecular mechanism of male infertility , and look for a new and effective treatment.

英文关键词： wuziyanzong pill;oligoasthenospermia;mitochondrial permeability pore;mechansim