新型长循环“双靶点”双模态分子探针对乳腺癌的可视化活体成像研究

项目名称： 新型长循环“双靶点”双模态分子探针对乳腺癌的可视化活体成像研究

项目编号： No.81201087

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 杨蕊梦

作者单位： 广州医科大学

项目金额： 23万元

中文摘要： 早期诊断对乳腺癌的治疗及预后具有重要意义。多模态分子影像为乳腺癌的早期诊断提供了新的视角。分子影像诊断依赖于灵敏度及特异性高的分子探针。由于肿瘤细胞表面受体表达不稳定，传统针对某单一靶点的分子探针面临肿瘤摄取低、体内药代动力学特性不佳等缺点。鉴于乳腺癌肿瘤高表达GRPR及integrin αvβ3，设计针对这两个肿瘤特异性受体的RGD-BBN双靶点分子探针具有独特优势。我们前期合成了长循环SPIO脂质体纳米颗粒，实验显示其物理性能较好，粒径小，分布均匀及体内外均具抗巨噬细胞吞噬功能。本课题在此基础上，将其核心SPIO与无镉量子点QDs组装，再在脂质体表面与肿瘤双靶向功能特异性受体RGD-BBN偶联，制备长循环肿瘤双靶点磁性/荧光脂质体双模态纳米分子影像探针，在乳腺癌动物模型上行磁共振及荧光成像研究，评价其对乳腺癌早期诊断的优势和可行性。一旦成功，对其他肿瘤受体多模态成像也有重要的借鉴作用。

中文关键词： 磁共振成像；分子影像；量子点；荧光成像；乳腺癌

英文摘要： Early diagnosis is important for the treatment and prognosis of breast cancer. Multimodal molecular imaging provides a new platform for the early diagnosis of breast cancer. Molecular imaging depends on the sensitive and specific molecular probe. Traditional molecular probes targeting single target has the disadvantages of low intake and undesirable pharmacokinetic properties, due to unstable expression of tumor cell receptors . Considering the breast cancer tumor with high expression of integrin αvβ3 and GRPR, it is superior to design RGD-BBN dual-receptor targeting molecular probe. In our previous basic research, we have synthesized SPIO coated with long circulating liposomes, and the experimental results showed that it was with the proper physical properties, particle sizes, uniform distribution and of anti-macrophage function in vitro and in vivo. Based on the results, we intend to use the long-circulating liposomes with excellent biological properties as a carrier, coating SPIO@QD800(InAs/InP/ZnSe) nanoparticles, and then coupling dual-receptor targeting RGD-BBN heterodimer for tumor, in order to prepare new long-circulating, dual-receptor targeting, magneto-optical dual-mode nanoparticles. Magnetic resonance and fluorescence imaging were performed on the animal models with breast cancer with the molecular

英文关键词： Magnetic resonance imaging；Molecular Imaging；quantum dots；fluorescence imaging；breast cancer