基于磁共振成像技术研究RKIP对ACh的调控在微波辐射致海马认知功能损伤中的作用

项目名称： 基于磁共振成像技术研究RKIP对ACh的调控在微波辐射致海马认知功能损伤中的作用

项目编号： No.81472951

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 左红艳

作者单位： 中国人民解放军军事科学院军事医学研究院

项目金额： 70万元

中文摘要： 电磁辐射损伤的临床影像学证据尚属空白，磁共振成像是当代影像学和神经科学研究的前沿领域。我们前期研究发现，RKIP参与了微波辐射致海马认知功能损伤的病理过程，但其作用机制未明。由于RKIP对学习记忆关键递质ACh合成具有重要调控作用，我们推测此调控对于微波辐射所致海马损伤具有重要意义。为此，本项目拟通过自主研发微波-磁共振融合技术，建立微波辐射致海马损伤大鼠模型，利用结构、功能和频谱磁共振成像研究海马损伤的影像学特征；在建立宏观磁共振影像表征同行为认知活动、微观组织病理及生物代谢之间联系的基础上，研究海马RKIP及其产物HCNP、ACh合成与代谢关键酶ChAT、AChE及受体nAChR表达和活性改变；最后，应用反义寡核苷酸技术通过海马立体定位注射深入探讨RKIP对ACh的调控在辐射损伤中作用。这将进一步揭示微波辐射脑损伤机制，并为其防治提供新思路，同时提供一种全新的临床影像学评价体系。

中文关键词： 微波辐射；磁共振成像；海马；乙酰胆碱；RKIP

英文摘要： Clinical imageology evidence is still vacant in the study of injury caused by electromagnetic radiation. Magnetic resonance imaging is the advancing front of imageology and neuroscience research. In previous studies, we found that RKIP participated in the pathological process of hippocampal recognition injury caused by microwave radiation. However, the mechanisms of RKIP is unclear. Because of the important role of RKIP in regulation of ACh synthesis, which is crucial for learning and memory, we postulate that this regulation may be important for hippocampus injury induced by microwave radiation. In this study, we will designed the combination of microwave and magnetic resonance independently, and established rat model injured by microwave radiation. Structural, functional and spectra imaging of magnetic resonance will be used to study characteristics of hippocampal injury. Based on the association of magnetic resonance imaging, behavior, pathology and biological metabolism, the expression or activation of enzymes and receptors related to ACh synthesis and metabolism such as ChAT, AChE and nAChR will be detected. Also, the expression of RKIP and its product HCNP will be examined. Finally, antisense oligonucleotide technique will be taken by hippocampal injection for further investigate the role of RKIP in ACh regulation. This study will further illuminate the mechanisms of brain injury induced by microwave radiation, and provide a new pathway for prevention and cure. Simultaneously, this study will provide a new imageological evaluation system for clinical research on microwave radiation injury.

英文关键词： microwave radiation;magnetic resonance imaging;hippocampus;acetylcholine;RKIP