拟南芥中蓝光与植物激素茉莉酸信号互作的分子机制研究

项目名称： 拟南芥中蓝光与植物激素茉莉酸信号互作的分子机制研究

项目编号： No.31470375

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 植物学

项目作者： 朱自强

作者单位： 南京师范大学

项目金额： 80万元

中文摘要： 蓝光与植物激素茉莉酸都对植物的生长、发育、防御病虫害以及衰老起重要调节作用。文献报道茉莉酸信号通路中的转录因子MYC2是蓝光信号通路中的负调控因子，蓝光受体CRY1和CRY2突变后植物会出现抗病以及衰老等茉莉酸相关表型，说明蓝光和茉莉酸存在信号交叉，但分子机制尚不清楚。在茉莉酸信号通路中，JAZ蛋白家族成员与MYC2互作并抑制MYC2的功能，我们初步实验结果发现蓝光受体CRY2在蓝光下至少可以和其中一个JAZ蛋白 (JAZ9) 直接互作，而在暗中不互作，推测JAZ蛋白可能是蓝光和茉莉酸信号的交叉节点，蓝光通过增强JAZ的活性抑制MYC2，促进光响应基因表达，而茉莉酸通过促进JAZ蛋白的降解活化JAZ结合的转录因子，实现茉莉酸的生理功能。本申请将研究蓝光下CRY-JAZ互作对JAZ蛋白生化活性的影响，以及整合蓝光和茉莉酸信号通路的分子机制。

中文关键词： 茉莉酸；蓝光；信号转导

英文摘要： Blue light and phytohormone jasmonate regulate plant growth, development, defense and senescence. It is reported that one jasmonate-activated transcription factor (MYC2) is a negative regulator in blue light signaling, while mutation of blue light photoreceptor CRY1 or CRY2 causes jasmonate related pathogen defense or senescence phenotype, suggesting that blue light and jasmonate signaling pathways are integrated at somewhere, although not clear yet. JAZ proteins interact and repress MYC2 functions in jasmonate signaling pathway. Our previous work found that CRY2 interacts with at least one JAZ protein (JAZ9) specifically in blue light but not in dark. We propose that JAZ proteins serve as an integration node for blue light and jasmonate signaling. Blue light enhances JAZ activities to suppress MYC2, so as to activate light responsive gene expression, while jasmonate promotes JAZ degradation and release other JAZ-interacted transcription factors to accomplish jasmonate-activated responses. We will study how does CRY-JAZ interaction affect JAZ proteins' biochemical activities and investigate the molecular mechanism for the integration of blue light and jasmonate signaling.

英文关键词： jasmonate;blue light;signal transduction