非接触式电容耦合电导检测微系统用于核酸扩增过程的实时定量检测

项目名称： 非接触式电容耦合电导检测微系统用于核酸扩增过程的实时定量检测

项目编号： No.21275153

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 金庆辉

作者单位： 中国科学院上海微系统与信息技术研究所

项目金额： 78万元

中文摘要： 致病微生物的快速检测和分析是公共安全突发事件现场迫切需求的技术，实时荧光定量PCR技术为微生物的快速鉴定提供了有效的手段，但目前都采用光学检测，仪器构造复杂，难以小型化，不能满足现场检测的需求。本项目提出基于MEMS技术制备集成非接触式电导检测器的核酸扩增微芯片系统，制作百纳米级SiO2/氮化硅介电层和精确对数的叉指电极，采用外源激励和差分信号读出方法,可显著提高电容耦合非接触电导检测的灵敏度、分辨率和抗干扰能力。本项目将在解决PCR扩增产物的非接触电导法特异性识别等关键问题的基础上，建立PCR反应过程的非接触式电导实时定量检测方法，构建手持式核酸快速检测分析仪样机，用于实际样本的分析和验证，实现在几分钟内微生物的现场快速鉴定和分析。本项目的研究成果将为食品安全、环境监测，以及公共卫生突发事件现场微生物的快速检测提供技术，具有重要的应用价值和社会意义。

中文关键词： 非接触式电导检测；PCR；微芯片；实时定量；

英文摘要： The rapid detection and analysis of pathogenic micro-organisms is the urgent needs of public safety emergency scene technology. Real-time quantitative PCR technique provides an effective means for the rapid identification of microorganisms. But it uses the optical detection apparatus and the structure is more complicated. So it is difficult to miniaturization and can not meet the needs of field testing. This project provides a novel microsystem, which integrate contactless conductivity detector for nucleic acid amplification real-time mornitoring and analysis. The microchip has one hundred nanometer level SiO2/silicon nitride dielectric layer and precision of the number of interdigital-electrodes. The readout circuit has the functionals of exogenous sitimulating and differential signal collection, it can significantly increase the capacitively coupled contactless conductivity detector sensitivity, resolution and anti-jamming capability. The project will reveal the PCR product specific recognition by the non-contact conductivity method.Based on the establishment of real-time quantitative detection methods for non-contact conductance of the PCR reaction. build hand-portable propetype analyzer for analysis and verification of the actual sample, for rapid identification and analysis of microorganisms within a few m

英文关键词： contactless conductivity detector；PCR；Microchip；Real-time quantitative；