microRNA-26a在压力超负荷心力衰竭中的作用和机制研究

项目名称： microRNA-26a在压力超负荷心力衰竭中的作用和机制研究

项目编号： No.81201453

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学四处

项目作者： 张振辉

作者单位： 广州医科大学

项目金额： 23万元

中文摘要： MicroRNAs (miRNAs)参与心力衰竭(HF)的发病，调控miRNAs的表达可能影响HF的进程。我们前期研究结果显示过表达miR-26a能抑制原代心肌细胞肥大和成纤维细胞胶原合成，且与糖原合成酶激酶3β(GSK3β)有关，但miR-26a对HF过程的影响以及具体调控机制仍有待进一步完善。本研究是基于前期研究的动物体内实验，拟采用腹主动脉缩窄(TAAC)建立压力超负荷大鼠和小鼠HF模型，应用超声引导腺病毒载体心肌注射和miRNA寡核苷酸静脉注射两种方法在动物心肌组织中过表达miR-26a，用心脏彩超仪和组织形态学方法观察miR-26a对HF的影响，并探讨miR-26a两个靶基因GSK3β和Enhancer of Zeste homolog 2（EZH2）与心肌细胞大小、细胞凋亡、心脏间质纤维化之间的关系。研究结果对阐明miR-26a在HF调控中的作用和机制以及临床应用有重要意义。

中文关键词： 微小RNA；心力衰竭；糖原合成酶激酶3β；；

英文摘要： MicroRNAs (miRNAs) comprise a broad class of small non-coding RNAs that control expression of complementary target messenger RNAs. Growing evidences indicates that miRNA expression is altered in human heart failure, and specific miRNAs has been linked to cardiac development and function. Our previous studies have demonstrated that overexpression of miR-26a regulates the expression of glycogen synthase kinase 3-beta (GSK-3β) in cardiaomyocyte and cardiac fibroblasts in vitro, which has impacts on cardiac hypertrophy and collagen synthesis. But effects and mechanisms of miR-26a on heart failure still need further investigation. Our study will focus on the role of miR-26a in rat and mouse models of transverse abdominal aorta constriction (TAAC)-induced pressure overload-heart failure. We first constructed miR-26a overexpression models in vivo by infecting TAAC animals with miRNA oligonucleotid intravenous injecting or with an adenoviral vector containing miR-26a precursor sequence intra-cardiomyocyte injecting under ultrasound guiding. The global cardiac structure and function will be analyzed by small animal echocardiography instrument and histomorphology. Our study also further investigates the mechanisms of miR-26a on heart failure. The expression levels of miR-26a and the target genes of miR-26a, GSK3β and Enha

英文关键词： MicroRNA；heart failure；glycogen synthase kinase 3β；；