AMPK-SIRT1/mTOR能量信号通路与肉鸡骨骼肌热应激效应关系及EGCG调控机制

项目名称： AMPK-SIRT1/mTOR能量信号通路与肉鸡骨骼肌热应激效应关系及EGCG调控机制

项目编号： No.31472117

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 杨烨

作者单位： 长江大学

项目金额： 80万元

中文摘要： 热应激引起骨骼肌能量代谢发生变化的同时，也引起细胞热应激效应，这是热应激使家禽生产性能下降的主要原因。热应激所诱导的骨骼肌能量代谢变化与热应激效应的关系及其分子机制是什么？本项目拟以肉鸡骨骼肌卫星细胞和AA型肉鸡为研究材料，首先研究了热应激引起的骨骼肌AMPK-SIRT1/mTOR能量信号通路变化与细胞热应激效应（线粒体生物合成、ROS产生、抗氧化体系变化、HSP表达及细胞氧化程度）的关系，其次使用特异抑制剂、RNAi技术分析了AMPK-SIRT1/mTOR信号通路及其上游关键因子通过磷酸化或去乙酰化对热效应目的基因的调节机制，并使用免疫共沉淀和启动子报告基因分析技术研究了热应激对AMPK、PGC-1α表达及其启动子活性的影响，通过这些研究阐明了AMPK-SIRT1/mTOR能量信号通路调控细胞热应激效应的分子机制。同时也证明EGCG正是通过该能量信号通路缓解家禽骨骼肌热应激。

中文关键词： AMPK-SIRT1/mTOR信号；热应激效应；肉鸡骨骼肌；EGCG；调控机制

英文摘要： The heat stress induces the change of energy metabolism from growth to maintenance and causes the heat stress response, which is the main reason of decreasing production in poultry. And what does the molecule mechanism between change of energy metabolism and heat stress response? This study clarifies the relationship between varies of AMPK-SIRT1/mTOR signal pathway and heat stress response (mitochondrial biogenesis, ROS production, change of antioxdative system, HSP expression and cell oxidation) induced by heat stress in the skeletal muscle satalite cell and AA chicken. This research also elucidates the mechanism of phosphorylation and deacetylation of upstream and AMPK-SIRT1/mTOR signal pathway regulating target gene related to heat stress response with the methods of specific inhibitors and RNAi technology. This study also analysis the effects of heat stress and EGCG on the expression and promotor activity of PGC-1α and AMPK with the method of co-immunoprecipitation and reporter gene assay. The above research conclusions show the molecule mechanism of which AMPK-SIRT1/mTOR signal pathway regulates the heat stress response in cell. EGCG also enhances the functions of self-guard via energy signal pathway, which contribute to alleviate the heat stess in the skeletal muscle satalite cell and AA chicken.

英文关键词： AMPK-SIRT1/mTOR signal;Heat stress response;Chicken skeletal muscle;EGCG;Regulation mechanism