阿尔茨海默病生物标志物的电化学发光成像分析

项目名称： 阿尔茨海默病生物标志物的电化学发光成像分析

项目编号： No.21505159

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 高分子科学

项目作者： 许林茹

作者单位： 中国药科大学

项目金额： 20万元

中文摘要： 目前对于阿尔茨海默病（Alzheimer’s Disease，AD）的早期诊断尚无准确快速的检测技术，成为制约有效防控AD的瓶颈。电化学发光成像技术可实现高灵敏度、高通量、多信息化和可视化检测，为AD早期诊断生物标志物的检测提供了新的途径。本项目拟在酶联免疫分析的原理基础上，结合纳米材料、生物信号放大系统和微阵列加工技术，采用电化学发光成像对血浆中3种AD生物标志物进行多组分同时检测。利用纳米材料包埋一抗，构筑导电性好、生物相容性佳的电化学传感界面。引入双酶或HRP模拟酶等新型生物信号放大技术，提高二抗与酶信号复合物的检测灵敏度。构筑多通道阵列式检测系统，利用电化学发光成像对所有阵列同时进行记录成像。本项目旨在实现血浆中AD标志物的灵敏准确检测，推动AD早期诊断技术向自动化、微型化及多组分检测方向发展。

中文关键词： 电化学发光成像；阵列传感器；阿尔茨海默病；早期诊断；生物标志物

英文摘要： The lack of early diagnosis of Alzheimer’s disease (AD) becomes critical bottleneck that hinders the effective treatment. Electrochemiluminescence (ECL) imaging is an effective method with the properties of high-sensitivity, high throughput, multi-informatization and visualization, providing a new way for the early diagnosis of AD via the determination of biomarkers in human plasma. The project aims to develop a multiplexed ECL imaging system combining nano materials, biological signal amplification system and microarray processing technique, for the simultaneous detection of 3 AD biomarkers based on enzyme immunoassay. Primary antibodies immobilized on nano materials can be used to construct the electrochemical biosensing interface with high conductivity and biological compatibility. The sensitivity of secondary antibodies/enzyme complex can be increased by introducing the biological signal amplification system involving bienzyme or HRP-mimicking DNAzyme. ECL imaging system consisting of multiplexed arrays enables simultaneous recording of the light from all arrays. This project provides an effective mode for the sensitive and accurate determination of AD biomarkers in plasma, which could make the early diagnosis of AD more automatic, miniature and multi-functional.

英文关键词： Electrochemiluminescence Imaging;Array Sensor;Alzheimer’s Disease;Early Diagnosis;Biomarker