旋毛虫副肌球蛋白H-2d限制性Th表位的鉴定及Th-B表位肽嵌合疫苗免疫学效应研究

项目名称： 旋毛虫副肌球蛋白H-2d限制性Th表位的鉴定及Th-B表位肽嵌合疫苗免疫学效应研究

项目编号： No.81201312

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学四处

项目作者： 顾园

作者单位： 首都医科大学

项目金额： 23万元

中文摘要： 旋毛虫病是一种常见的人畜共患寄生虫病，严重危害人类健康和畜牧业生产。旋毛虫副肌球蛋白（Ts-Pmy）是本课题组新发现的一个具有应用前景的抗旋毛虫病疫苗候选抗原分子，其重组蛋白对免疫小鼠具有一定保护性。宿主的体液免疫对旋毛虫的清除十分重要，因此鉴定Ts-Pmy 的Th表位并研究其特异性的免疫应答特征具有重要的意义。本课题拟以BALB/c小鼠为模型，首先采用生物信息学预测Ts-Pmy的H-2d限制性Th表位，体外固相合成表位肽，然后结合CD4+T淋巴细胞功能实验和细胞因子检测等方法确定Th2表位；然后将筛选出的Th2表位和本课题组前期鉴定的Ts-Pmy中一个具有保护性的B细胞表位串联构建Th-B表位肽嵌合疫苗；将此多抗原肽（MAP）疫苗免疫BALB/c小鼠，分析其所引起的细胞和体液免疫反应；攻虫后计算减虫率，评估疫苗的免疫效果。本课题将为旋毛虫病表位疫苗的设计提供实验依据和奠定基础。

中文关键词： 旋毛虫；CD4+ T 细胞；表位；疫苗；

英文摘要： Trichinellosis is a popular food-borne zoonosis which is threatening human health and causes great loss of livestock production. Our group has reported a newfound promising vaccine canditate, paramyosin (Pmy) of Trichinella spiralis (Ts-Pmy), exhibits protective immunity effect in mice. It has been studied that host humoral immunity is important in the elimination of the parasite. Furthermore, it is necessary to indentify T helper (Th) epitope of Ts-Pmy to specify its immune response caused accordingly. Based on BALB/c mice model, we are planning to predict H-2d restricted Th epitopes of Ts-Pmy by bioinformatics approach and prepare those epitope peptides by solid-phase procedure. Their function will be verified through CD4+ lymphocyte proliferation assays and cytokine production induced by the peptides. New-discovered Th2 epitope(s) will then be constructed in tandem with the protective B epitope of Ts-Pmy which has been identified by our group previously. Based on that, a chimeric Th-B vaccine is able to be constructed. The humoral and cellular immune responses against this multiple antigen peptide (MAP) will be studied in BALB/c mice. Larvae burden reduction will be calculated to evaluate the protective immunity of this new antigen in Trichinella spiralis challenged animals. If the antigen is confirmed to wor

英文关键词： Trichinella spiralis；CD4+ T cell；epitope；vaccine；