‘黄花’梨及其绿皮芽变果皮色泽发育相关基因克隆与功能研究

项目名称： ‘黄花’梨及其绿皮芽变果皮色泽发育相关基因克隆与功能研究

项目编号： No.31272140

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 乔玉山

作者单位： 南京农业大学

项目金额： 80万元

中文摘要： 砂梨果实褐皮和绿皮性状相关基因克隆及其功能研究对果皮色泽分子育种具有重要意义，'黄花'梨及其绿皮芽变'绿黄花'为该研究提供了理想的试材。我们已找出了该对材料一些差异表达基因，如PAL、C4H、CCR，至少是苯丙烷代谢相关基因表达发生了改变。为了进一步研究褐皮和绿皮性状相关基因及其功能，本项目以克隆果皮色泽发育相关基因及其在色泽发育过程中的功能为主要研究内容，一方面通过转录组测序对果皮色泽发育关键期差异表达基因进行更全面的分析；另一方面利用GC-MS技术，分析果皮表皮差异代谢产物并与差异表达的基因进行比照分析确定出候选基因，克隆基因全长序列，构建其正义/反义表达载体，利用农杆菌微注射基因瞬时表达技术研究这些基因在果皮色泽发育过程中的作用，同时筛选重要的基因转化番茄深入研究其功能。预期结果是探明控制褐皮/绿皮发育的关键基因及其作用，为果皮色泽分子育种提供重要的功能基因。

中文关键词： 黄花梨；芽变；褐皮；差异基因；基因功能

英文摘要： Study on genes involved in russet or green fruit traits and their functions is very significant for molecular breeding of fruit color on sand pear (Pyrus pyrufolia Nakai), and Pyrus pyrufolia Nakai 'Huanghua' and its green skin bud sport (tentatively named as 'Green Huanghua') provide perfect material for carrying out this research. We have found some differentially expressed genes between 'Huanghua' and 'Green Huanghua' fruit peels, including phenylalanine ammonia lyase gene (PAL), cinnamic acid 4-hydroxylase gene (C4H) and cinnamoyl-CoA reductase gene (CCR), which suggest expression level of genes involved in phenylpropane metabolism is changed between the two materials. In order to further study genes related to russet and green fruit traits, in this project we will clone genes related to fruit color development from 'Huanghua' and 'Green Huanghua' pear fruit and study their roles in fruit color formation. On the one hand, comprehensive analysis of differentially expressed genes between 'Huanghua' and 'Green Huanghua' fruit peel at the key developmental stage of fruit color would be performed by transcriptome sequencing; On the other hand, GC-MS is used to analyze and identify the different metabolites between peel epidermises of the two pear fruits, which are used to select the key genes involving in fruit c

英文关键词： Pyrus pyrifolia 'Huanghua'；Sports；Russet skin；Differentially gene；Gene function