纳流体环境下单个DNA分子的过孔动力学研究

项目名称： 纳流体环境下单个DNA分子的过孔动力学研究

项目编号： No.51306037

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 能源与动力工程

项目作者： 孙东科

作者单位： 东南大学

项目金额： 25万元

中文摘要： 本项目针对第三代DNA测序研究中的关键问题，综合采用数值模拟和实验研究两种手段，研究纳流体环境下单个DNA分子的动力学行为，探索DNA与外场之间的相互作用机理，具体而言：(1) 根据格子玻尔兹曼方法、布朗动力学方法和浸入式边界方法的基本原理，建立研究纳流体环境下DNA动力学行为的多场耦合数值模型与高性能模拟方法；(2) 研制基于硅基/碳基纳米孔复合制备工艺的微纳复合通道，搭建操控与表征DNA过纳米孔行为的实验装置；(3) 通过数值模拟与实验研究，定量研究DNA纳尺度流场、电场和温度场共同作用下的动力学行为，揭示DNA运动行为响应外场变化的机制。本项目对进一步发展DNA纳流动力学理论体系具有重要意义，将为设计和制造低成本、高性能、高可靠性的DNA测序仪器提供理论支撑和技术支持。

中文关键词： 生物粒子；微流动；格子玻尔兹曼方法；布朗动力学方法；数值模拟

英文摘要： In the project, numerical simulation and experimental investigations on the single DNA molecules translocation through a nanopore in nanofluids will be carried out aiming to solve the key problems in the study of the 3rd-generation DNA sequencing, and the dynamics mechanism of the single DNA molecules in nanofluids and the interactions between the DNA and external fields will be explored, particularly: (1) According to the basic principles of lattice Boltzmann method, Brownian dynamics method and immersed boundary method, an originality multi-fields coupled model will be established, and the high-performance algorithms will be developed subsequently; (2) A silicon/carbon-based micro- and nano- composited channel will be manufactured, and an experimental apparatus for manipulation and characterization of DNA translocation through a nanopore will be built; (3) Numerical simulation and experimental studies on the transport process of DNA in nanofluids will be performed, especially in studying the dynamic behavior of DNA under the nanoscale flow field, thermal field and electronic field environment and the mechanism of DNA's dynamic behavior responding to the changes of external fields. This project is of great importance in further developing the nanoscale fluid dynamics of DNA, and it will provide theoretical a

英文关键词： Bioparticle；Microflows；Lattice Boltzmann method；Brownian dynamics method；Numerical simulation