基于代谢组学系统评价水体中不同性质微塑料颗粒的生物毒性效应研究

项目名称： 基于代谢组学系统评价水体中不同性质微塑料颗粒的生物毒性效应研究

项目编号： No.21507058

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 化学工业

项目作者： 张宴

作者单位： 南京大学

项目金额： 21万元

中文摘要： 微塑料在水体中的大量积累，威胁环境健康和生态安全，其对生物体的毒性效应备受关注。微塑料易被生物体摄入，并在体内富集产生毒性，但其富集规律和致毒机理尚不明确，缺乏系统全面的解析。我们初步探索发现，微塑料在动物不同组织器官的富集过程存在差异，此外微塑料还可导致动物血清代谢组紊乱，相关研究未见报道，有必要开展深入研究。本项目拟以水体中不同性质（成分、粒径和表面电性）的微塑料颗粒作为研究对象，借助荧光标记技术和代谢组学技术，系统研究水体中不同性质微塑料颗粒的生物毒性效应。研究旨在揭示微塑料颗粒在动物体内的富集、分布和排泄规律；深入剖析微塑料颗粒的生物毒性及其影响因素；阐明不同性质的微塑料颗粒对生物体毒性作用的微观机理，并筛选相应的敏感的分子生物标志物，为系统评价水体中微塑料的健康风险提供理论指导与科学依据。

中文关键词： 微塑料；水体；毒性效应；代谢组学；分子生物标志物

英文摘要： Large accumulations of microplastics in the water threaten the environmental health and ecological safety. The toxic effects of microplastics to organisms receive widespread attention. Microplastics can be easily ingested by organisms and enrich in vivo, which result in toxic effects. However, the enrichment regulation and the toxicity mechanism are unknown. Systematic and comprehensive analysis is still very rare. Through initial exploration, we found that the enrichments of microplastics are different in different tissues, and metabolomic disorders were also induced by microplastics exposure. However, these phenomenons have not been reported, and further research is needed. This project intends to choose different properties (composition, particle size and surface charge) of microplastics as target compounds. Fluorescent labeling technology and metabolomics technology will be used to study the toxic effects of microplastics. This research aims to reveal the regulation of enrichment, distribution and excretion of microplastic in vivo, study the toxic effects of microplatics and its influence factors, elucidate the toxic mechanisms and discover sensitive biomarkers. The results of this project will provide theoretical guidance and scientific basis for the health risk assessment of microplastics.

英文关键词： microplastics;water;toxic effects;metabolomics;molecular biomarkers