双酚S作用脂质代谢途径的关键因子及机理研究

项目名称： 双酚S作用脂质代谢途径的关键因子及机理研究

项目编号： No.31501569

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 周兴华

作者单位： 江苏大学

项目金额： 20万元

中文摘要： 由于双酚A的内分泌干扰作用，使其在食品包装材料中的应用受到限制，取而代之的双酚S被广泛采用。然而双酚 S 在大规模应用之前，并没有对其毒性进行充分研究。体外实验表明，低剂量的暴露水平下，双酚S能降低脂肪细胞的分解速度，引起甘油三酯在脂肪细胞内大量积累，但是目前双酚S对脂质代谢的报道极少，且未见BPS在体内代谢的相关研究。本课题通过研究BPS干扰脂质代谢作用途径，基于生化及能量代谢理论，构建细胞及动物实验模型，采用HPLC-MS-MS、荧光定量PCR技术、透射电镜、酶联免疫和Western blot 等手段，对3T3-L1 前脂肪细胞的增殖分化及大鼠血清、脂肪、肝脏、粪便等进行相关指标检测，分析BPS干扰脂质代谢的作用途径及关键因子，进一步揭示BPS促进肥胖的作用机理及本质原因，为BPS在食品包装材料中的安全使用提供科学判据和参考。

中文关键词： 双酚S；脂质代谢；肥胖；食品包装；安全

英文摘要： Since bisphenol A (BPA) is restricted used in a variety of food packaging materials for its endocrine disruption, bisphenol S (BPS) is widely used. But People use BPS to replace BPA without sufficient toxicological information. In vitro study the BPS can induce a decrease in lipolysis at low doses, it has produced an increase in triglycerides in adipocytes. However, there is no research on BPS metabolism in vivo. On the basis of biochemical and energy metabolismtheory, BPS interfering with lipid metabolism pathway is investigated, in order to construct the cell and animal models. HPLC-MS-MS, fluorescent quantitative PCR technology, transmission electron microscopy, ELISA and Western blot are used for the study of followings, including the tests of proliferation and differentiationof 3T3-L1 preadipocytes, rat serum, fat, liver and so on, by analyzing the paths and key factors for BPS interfere with lipid metabolism, thus can reveal themechanism and essential reasons of the BPS-induced obesity. The scientific criterion and technical basis are provided in the BPS safety for using in food packaging materials.

英文关键词： Bisphenol S;lipid metabolism;obesity;food packaging;safety