假交替单胞菌SCSIO11900鞭毛介导运动性在微生物被膜形成中的作用

项目名称： 假交替单胞菌SCSIO11900鞭毛介导运动性在微生物被膜形成中的作用

项目编号： No.31500093

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 蔡兴盛

作者单位： 中国科学院南海海洋研究所

项目金额： 20万元

中文摘要： 海洋微生物种类繁多，能够适应多变的海洋环境，且能够产生多种活性物质，已经越来越受到重视。假交替单胞菌属分布广泛，且普遍能够形成微生物被膜，是研究海洋细菌微生物被膜的理想模型。本文以来源于三亚4米深的珊瑚表面的细菌假交替单胞菌SCSIO11900（11900）为研究对象，基于本实验室前期的研究基础，着眼于11900的鞭毛系统和微生物被膜。通过荧光定量PCR和western bolt对11900鞭毛基因mRNA和蛋白水平的微生物被膜不同阶段的表达差异初步阐述鞭毛与微生物被膜的关系。通过基因敲除、回补研究鞭毛结构基因和运动性相关基因，初步探讨鞭毛和鞭毛介导运动性在在微生物被膜形成中作用。通过扫描电镜和荧光共聚焦显微镜比较野生菌株和突变菌株在微生物被膜结构上的差异，阐述鞭毛以及运动性对于微生物被膜结构的影响。

中文关键词： 海洋微生物；鞭毛；运动性；微生物被膜

英文摘要： Marine microbes are well known for their diversity, their associated with variety function in bioactive production and their adaption to the extremely marine environments. Pseudoalteromonas genus has gained increased attention for their broad distribution and adaptability to different the marine environments. Pseudoalteromonas genus is an ideal model to study biofilm of marine bacteria for they can form a great mount of biofilm and pellicle. In this study, we focused on Pesudoalteromonas sp. SCSIO11900 (11900), a gram negative bacterium isolated from the surface mucus layer of the coral at 4 m deep in the South China Sea. Basing on our previously results on 11900 biofilm, we aim to explore relationship of motility mediated by flagella and biofilm. We will study roles of flagella system in biofilm through comparing expression of flagella in both mRNA and protein level in different stages biofilm. The physiological function of flagella structure and motility mediated by flagella will be assessed through knockout and complementary study of certain genes. Moreover, function of flagella and motility in biofilm structure will be illustrated through scanning electron microscope and confocal microscopy studies.

英文关键词： pseudoalteromonas;flagella;motility;biofilm