基于碳纳米材料电化学发光猝灭效应的生物传感平台构筑及应用研究

项目名称： 基于碳纳米材料电化学发光猝灭效应的生物传感平台构筑及应用研究

项目编号： No.21505128

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 高分子科学

项目作者： 刘中原

作者单位： 中国科学院长春应用化学研究所

项目金额： 21万元

中文摘要： 碳纳米材料具有良好的生物相容性和低毒性，常被作为载体、电极修饰材料或信号放大元件应用于电化学发光传感器中。然而，将碳纳米材料作为电化学发光猝灭剂应用到电化学发光体系中的相关研究较少。本项目拟开展不同碳纳米材料对电化学发光体系猝灭效应研究。在深入研究碳纳米材料及功能化碳纳米材料的电化学发光猝灭效率与碳纳米材料结构及外部环境相互关系的基础上，揭示电化学发光猝灭机理，并筛选出具有高猝灭效率的碳纳米材料、功能化碳纳米材料和相应的电化学发光体系。基于筛选出的碳纳米材料电化学发光猝灭体系，结合碳纳米材料对DNA独特的吸附能力，构建高灵敏的电化学发光DNA和核酸适配体传感平台，实现对DNA、蛋白质或小分子等目标物的快速灵敏检测。该研究的开展将建立多种高灵敏电化学发光分析检测新方法，为碳纳米材料在电化学发光领域的应用提供一种新思路。这些研究有望促进电化学发光分析检测方法的发展，具有重要的研究价值。

中文关键词： 电化学发光；碳纳米材料；DNA；核酸适配体；生物传感器

英文摘要： Carbon nanomaterials possess good biocompatibility and low toxicity. These are usually used as a carrier, a modified material or signal amplification element in electrochemiluminescence (ECL) sensors. However, reports about using carbon nanomaterial as ECL quencher are quite rare. In this project, we are planning to study the quenching efficiencies of different carbon nanomaterials to ECL. Impact on the quenching efficiency from the structure of carbon nanomaterial as well as other external conditions will be studied. On this basis, ECL quenching mechanism will be revealed, and carbon nanomaterial with high quenching efficiency and corresponding ECL system will be selected. Since carbon nanomaterial has a unique DNA adsorption capacity, a series of DNA and aptamer sensing platforms based on the selected carbon nanomaterials-ECL quenching systems will be developed for the sensitive and specific detection of DNA, protein and small molecules. Through this project, new highly sensitive ECL detection method will be established, and that will provide a new attention for application of carbon nanomaterial in the field of ECL. This project will also promote the development of ECL analysis and detection methods, which will have important and attractive research value.

英文关键词： electrochemiluminescence;carbon nanomaterials;DNA;aptamer;biosensor