氮源等环境因子对皮状丝孢酵母形态转换的调控及其分子机制

项目名称： 氮源等环境因子对皮状丝孢酵母形态转换的调控及其分子机制

项目编号： No.21466015

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 颜日明

作者单位： 江西师范大学

项目金额： 53万元

中文摘要： 皮状丝孢酵母（Trichosporon cutaneum）为二型态真菌，其含油量高并可利用木质纤维素，是微生物油脂生产的重要候选菌种。在前期研究中，我们发现氮源可有效调控T. cutaneum的形态，成为影响发酵水平的关键。因此开展T. cutaneum细胞形态转换的环境调控因子及其分子机制研究不仅能揭示其形态转换规律，丰富二型态真菌形态转换理论，而且对于提高二型态真菌的发酵水平具有重要意义。但迄今有关T. cutaneum形态转换未见研究。本项目以T. cutaneum为对象，采用平板结合反应器恒化培养，探寻影响其形态转换的环境因子，并着重探讨氮源（氨基酸）对其形态的调控过程，在此基础上，利用抑制消减杂交（SSH）技术并结合表达序列标签（ESTs）分析，比较其酵母型和菌丝型间的基因表达差异，同时利用实时定量PCR技术进行验证，以揭示不同条件下T. cutaneum形态转换的分子机制。

中文关键词： 皮状丝孢酵母；形态转换；环境因子；调控机制；微生物油脂生产

英文摘要： The dimorphism fungi Trichosporon cutaneum was an important candidate for microbial oil production for its high oil content and can hydrolyze lignocellulose. Our previous study showed that nitrogen source can efficiently regulate the morphologic transition of T. cutaneum and it has become a key factor to affect the fermentation level. Therefore, probe the environmental factors and its regulatory mechanism for morphologic transition of T. cutaneum is significant no only to discover its morphologic transition pattern and enrich the morphologic transition theory of dimorphism fungi but also to improve the fermentation level. However, study on morphologic transition of T. cutaneum is rather insufficiency. In present study, we explored the environmental factors especially the nitrogen resource (amino acid) to regulate the morphologic transition of T. cutaneum in plate culture and chemostat culture, and on the basis of that we applied the technology of suppression subtractive hybridization (SSH) and expressed sequence tags analysis to compare the gene expression difference between the yeast and pseudohyphal forms of T. cutaneum cell. Additionally, the real-time quantitative PCR method was also used to check the differentially expressed gene so as to elucidate the molecular mechanism of morphologic transition of T. cutaneum under different environments.

英文关键词： Trichosporon cutaneum;morphologic transition;environmental factors;regulatory mechanism;microbial oil production