AEG-1对外周T细胞淋巴瘤恶性生物学行为的影响及机制研究

项目名称： AEG-1对外周T细胞淋巴瘤恶性生物学行为的影响及机制研究

项目编号： No.81500174

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 严家芹

作者单位： 郑州大学

项目金额： 18万元

中文摘要： 外周T细胞淋巴瘤治疗效果差，预后不良，目前发病机制不明。AEG-1是新近发现的一种癌基因，与多种肿瘤发生发展相关。该基因对外周T细胞淋巴瘤的影响如何？其机制是什么？国内外至今未见相关文献报道。本课题组前期研究发现：AEG-1在外周T淋巴瘤中高表达，与化疗敏感性、生存期、预后等临床指标有相关性。本项目拟在前期工作基础上：①进一步检测AEG-1在正常淋巴结组织和外周T细胞淋巴瘤组织及T淋巴瘤细胞系的表达情况；确定AEG-1的表达与外周T细胞淋巴瘤临床指标之间的关系。②通过体内、体外实验阐明AEG-1对外周T细胞淋巴瘤恶性生物学特征的影响及机制。③应用高通量技术检测不同表达水平AEG-1的T淋巴瘤细胞系与增殖、凋亡、耐药、细胞周期等相关的差异表达基因。本研究结果将可能阐明AEG-1在外周T细胞淋巴瘤中的作用和分子机制，并有望发现新的外周T细胞淋巴瘤预后标志和治疗靶点。

中文关键词： 淋巴瘤；外周T细胞淋巴瘤；星形细胞上调基因-1；基因表达；恶性生物学行为

英文摘要： Peripheral T-cell lymphoma is a high- incidence malignant disease, which is considered to be highly aggressive with high death rate. At resent, the pathogenesis of this disease is still unclear. AEG-1 is a newly identidied oncogene. Recent studies have implicated that AEG-1 plays an important role in the biological processes of many cancers. However the molecular mechanism of AEG-1 on peripheral T cell lymphoma remains unclear. Our previous studies show that AEG-1 is overexpressed in peripheral T-cell lymphoma. Patients with high AEG-1 expression had significantly poor overall survival and disease-free survival. In this application, we are attempting to investigate: ①the expression of AEG-1 in normal lymph nodes, peripheral T cell lymphoma tissues and T lymphoma cell lines. ②the malignant biological characteristics and mechanisms of peripheral T-cell lymphoma regulated by abberant AEG-1 expression via in vivo and in vitro studies..③the gene-expression profile related to proliferation, apoptosis, cell cycle, drug resistance and invasion under different AEG-1 expression using digital gene expression technique, which will be identified by Real-time PCR and Western blotting. This study will contribute to a better understanding of the AEG-1 gene and its targets, as well in understanding the role of this gene in T cell lymphoma occurrence and development, and will provide a new insight that targeting AEG-1 may be a promising therapeutic strategy for peripheral T cell lymphoma patients.

英文关键词： lymphoma;；Peripheral T-cell lymphoma;Astrocyte elevated gene-1;gene expression;malignant biological behaviors