小麦太谷核不育基因Ms2的图位克隆

项目名称： 小麦太谷核不育基因Ms2的图位克隆

项目编号： No.31471161

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 付道林

作者单位： 山东农业大学

项目金额： 80万元

中文摘要： 小麦是我国第二大粮食作物，小麦生产关系我国粮食安全。上世纪60年代，我国开始关注小麦雄性不育，用以挖掘杂种优势。1972年，山西太谷诞生了一株穗部蓬松、呈半透明状的小麦，后发展为国宝级种质太谷核不育小麦。太谷核不育受小麦 4DS 染色体上的显性基因 Ms2 控制，在轮回选择中发挥了重要作用。克隆太谷核不育基因 Ms2 是我国小麦工作者梦寐以求的夙愿。为此,我们配制了太谷核不育-鲁麦15 （LM15Ms2）和人工合成小麦SN421的 BC1F1 回交群体，借鉴小麦 D 基因组供体 - 山羊草的高密度图谱，充分挖掘麦类作物基因组数据，现已将 Ms2 对应区域锁定在山羊草的物理图谱中。目前研究的重点是构建 LM15Ms2 中 Ms2 区域的物理图谱，分离候选基因，确认候选基因的功能，并运用转录组测序（RNA-seq）揭示Ms2 基因的调控网络。本项研究具有重要的理论和应用价值。

中文关键词： 雄性不育；轮回选择；杂交小麦；RNA-Seq；Tal

英文摘要： Wheat is the No. 2 cereal crop impacting food security in China. As early as the last 60s, people started to pay attention to male sterile wheat which could be used to produce hybrid wheat. In 1972, one wheat plant whose spikes had wide-open glumes and appeared to be semitransparent in the sunlight was discovered in Taigu country in Shanxi province, which was later reconginzed as the national treasure known as Taigu genic male sterile wheat. The Taigu male sterility is controlled by the single dominant gene Ms2 on wheat chromosome 4DS, which has been used as a powerful tool in wheat recurrent selecction and has potential application for hyrid seed production in wheat. Cloning of Ms2 gene has been a long-term goal of wheat scientists in China. In the current study, we developed the BC1F1 population from the Ms2 carrierTaigu - Lumai 15 (LM15Ms2)and the hexaploid synthetic wheat SN421, took the advantage of the high-density maps of Aegilops tauschii (wheat D-genome donor) and Triticeae genome data to develop PCR markers in the Ms2 region, and located the Ms2 corresponding region in current physical maps of Ae. tauschii. Our goal is to construct the Ms2 physical map in LM15Ms2, idnetify Ms2 candidate genes, validate putative Ms2 candidates using transgenic complementation and/or gene scilencing, and characterize Ms2-regulated transcriptome using RNA-seq techniques. This study presents great value in science and production, and will be important for food security in China.

英文关键词： Male sterility;recurrent selection;hybrid wheat;RNA-Seq;Tal