调控三七皂苷生物合成转录因子的克隆与功能研究

项目名称： 调控三七皂苷生物合成转录因子的克隆与功能研究

项目编号： No.31260070

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 葛锋

作者单位： 昆明理工大学

项目金额： 48万元

中文摘要： 三七为人参属药用植物，全世界绝大部分的三七药材产于云南省。三七皂苷是三七药材的主要活性成分，以三七皂苷为主要成分的药品已成为防治心脑血管疾病的特效天然药物。由于三七种植对生境要求苛刻，病虫害严重，需要轮作，土地利用率低，难以扩大种植面积，导致供需矛盾日渐突出。为保证三七产业的可持续发展，需要借助现代生物技术手段解决药用资源短缺问题。因此，有必要开展与三七皂苷生物合成相关的基础研究工作。本课题以体外培养的三七细胞为研究对象，采用代谢工程研究思路，基于前期对三七皂苷生物合成途径关键基因的认识，以茉莉酸甲酯为诱导剂，采用酵母单杂交和同源法获得影响三七皂苷生物合成的转录因子，开展转录因子对皂苷合成途径关键基因表达的调控研究，明确转录因子在控制皂苷代谢流方面的功能和地位，充分发挥转录因子多点调控优势，为三七皂苷同源或异源高效表达系统的构建提供理论依据和技术参考。

中文关键词： 三七；转录因子；次生代谢；皂苷；代谢工程

英文摘要： Panax notoginseng is a kind of medicinal plant, which belongs to Panax L.. The production of P. notoginseng in Yunnan province occupies most of the total amount in the world. The main active ingredients in P. notoginseng are saponins. The medicines which are mainly composed of saponins of P. notoginseng have become the specific natural medicines in preventing and treating cardiovascular and cerebrovascular diseases. Because of the harsh growth environment, plant diseases and pests, crop rotation needing, low land-capability and difficult to popularize large cultivation of P. notoginseng, the gap between supply and demand has been broadened significantly. The basic research of the biosynthesitic pathway of P. notoginseng saponins needs to be strengthened in order to lay the foundation for solving the medicinal resource shortage by modern biotechnology and ensure the sustainable development of P. notoginseng. In this proposed project, the object is to culture P. notoginseng cells in vitro and metabolic engineering will be used. On the basis of understanding of the key genes in the biosynthesitic pathway of P. notoginseng saponins, methyl jasmonate will be used as inducer and the transcription factors will be obtained by the methods of yeast one-hybrid and homologous cloning. The expressions of key genes in the bio

英文关键词： Panax notoginseng；transcription factor；secondary metabolism；saponin；metabolic engineering