茶树酚类物质生物合成分支途径调控的分子机理

项目名称： 茶树酚类物质生物合成分支途径调控的分子机理

项目编号： No.31470689

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 夏涛

作者单位： 安徽农业大学

项目金额： 85万元

中文摘要： 本课题拟利用LC-MS/MS技术，建立茶树酚类物质种质资源、时空分布和诱导积累的代谢图谱；利用基因组、转录组和small RNA测序数据，寻找茶树酚类物质合成相关结构基因和转录因子、microRNA和microRNA靶基因；利用qRT-PCR或tag测序技术，构建上述相关基因种质资源、时空表达和诱导表达的特异性图谱；对代谢图谱和基因表达图谱进行关联分析，初步构建酚类物质合成代谢调控网络；利用茶树组织培养或幼苗培养平台，研究不同环境因子诱导或不同前体饲喂条件下，酚类物质分支途径关键节点的响应模式，初步验证构建的产物与基因、基因与基因之间、microRNA与基因之间的调控网络；通过原核表达、酶活测定及转基因手段，对关键节点的基因或microRNA的功能进行研究，进一步验证网络中各节点之间的调控关系。本课题旨在探寻茶树酚类物质生物合成分支途径调控的分子机理，揭示茶树酚类物质合成代谢的调控网络

中文关键词： 茶多酚；生物合成；代谢图谱；调控网络；分子机制

英文摘要： The accumulation modes of different tea polyphenols synthesized from branch pathways under different varieties, spatio-temporal and inducible expressions will be tentatively established by UPLC-3Q-MS/MS technology. These functional genes,transcription factor,microRNA and their target genes related to tea polyphenols biosynthesis will be screened out from the constructed genome and transcriptome database as well as by small RNA solexa. The expression patterns of those genes from different varieties, spatio-temporal and inducible expressions will be set up via qRT-PCR or tag solexa for mapping gene-expression profiles. Then the regulatory networks of tea polyphenols biosynthesis can be preliminarily constructed based on the correlation analysis between the metabolic pathways and gene expression profiles. To verify the functions of functional genes or microRNAs at the key branch nodes of the regulatory networks of tea polyphenols biosynthesis, their response modes under the different environmental factors or different precursor conditions will be investigated by tea tissue culture or seedling culture. The regulatory networks between constructed products and genes, genes and genes, microRNA and genes are going to preliminarily demonstrated. The relationships among key branch nodes of regulatory networks could be further explained in vivo or in vitro by using prokaryotic expression system, enzyme assay and transgenic technology. Our objective aims to investigate the molecular regulation on the branch networks of tea polyphenols biosynthesis.

英文关键词： tea polyphenols;biosynthesis;metabolic profile;regulatory networks;molecular mechanism