林麝化脓性疾病与细菌识别相关TLR基因表达及多态性的关系研究

项目名称： 林麝化脓性疾病与细菌识别相关TLR基因表达及多态性的关系研究

项目编号： No.81503188

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 竭航

作者单位： 重庆市药物种植研究所

项目金额： 18万元

中文摘要： 麝香为名贵中药，资源量少，供不应求。麝香重要基原动物林麝化脓性疾病的高发严重阻碍了林麝种群数量增长，极大限制了麝香产量的提高。Toll样受体（Toll-like receptors, TLRs）在病原微生物识别及感染信号传递中起重要作用，因此本项目提出：细菌识别相关TLR基因表达及多态性对林麝化脓性疾病的发生具有关键作用。.项目组掌握了“活麝取香”关键技术，已完成林麝化脓性疾病的临床症状观察、发病率统计、病理切片制作及样本采集等工作。本项目拟克隆林麝细菌识别相关TLR基因全序列，检测和分析同一种群中健康林麝和化脓性疾病易感林麝目的基因全序列的多态性，同时，研究化脓性疾病对林麝主要脏器中各目的基因表达量的影响，从表达及多态性两方面揭示目的基因与林麝化脓性疾病发生的关系。本项目为林麝的选种育种和提高林麝种群对化脓性疾病的抵抗力提供理论基础，对林麝资源的保护与名贵中药麝香产量的提高具有重要意义。

中文关键词： 林麝；化脓性疾病；TLR；基因；表达；多态性

英文摘要： Musk, a kind of precious Chinese traditional medicine, is too rare to meet the need. Forest musk deer is the original animal of musk. The high incidence of purulent diseases seriously hindered the increase of forest musk deer population and limited the yield of musk in production. Toll like receptors (TLRs) play an important role in pathogenic micro-organism recognition and the infection signal transduction. Thus, the project put forwards: the expression and polymorphisms of bacterial recognition related TLR genes play a key role in the occurrence of purulent diseases in forest musk deer.. We have mastered the key technology of artificial musk acquisition with forest musk deer alive. Additionally, we have completed the clinical symptom observation of purulent diseases, morbidity statistics, the pathological section making and sample collection. Therefore, the complete sequences, including exons and introns, of bacterial recognition related TLR genes in forest musk deer will be cloned. On this basis, the polymorphisms of target gene exon and intron sequences between the health and purulent disease susceptible forest musk deer will be detected and analyzed. Meanwhile, the effects of purulent diseases on the expression of these genes in main organs of forest musk deer will be detected using real-time PCR, to elucidate the relationship between the target genes and purulent diseases in the aspects of gene expression and polymorphisms. This project will provide a theoretical basis for forest musk deer breeding and improving the resistance to purulent diseases. It is very important in resource protecting of forest musk deer and increasing the yield of musk.

英文关键词： forest musk deer;purulent diseases;TLR genes;expression;polymorphisms