拮抗芽孢杆菌在根部定殖及扩展分子机制探究

项目名称： 拮抗芽孢杆菌在根部定殖及扩展分子机制探究

项目编号： No.31201556

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 乔俊卿

作者单位： 江苏省农业科学院

项目金额： 23万元

中文摘要： 研究表明，生防菌的防效与其在植物根表的定殖能力密切相关。生防菌能否有效定殖已成为其大规模商品化应用的重要制约因子。已有定殖研究主要集中于根围分泌物对生防菌的影响，不能全面、准确地反映生防菌参与定殖及扩展的相关分子机制。申请人基于对拮抗芽孢杆菌Bs916在番茄根部定殖研究结果，提出本研究内容，拟采用基因表达谱技术对Bs916在番茄根部定殖及扩展过程实时分析，结合全基因组测序平台，建立Bs916定殖及扩展相关基因数据库；采用芽孢杆菌分子生物学技术（启动子研究、基因敲除等）对定殖及扩展中的关键基因进行功能研究，明确其作用机制，从而丰富生防芽孢杆菌在根部定殖和扩展的分子机制。预期研究结果将能够更加实时，全面地反映出生防菌参与定殖及扩展相关基因的表达情况，为阐释定殖分子机制提供新思路和线索，同时也为改善生防菌定殖能力、提高防效提供靶标位点和理论依据。

中文关键词： 芽孢杆菌；定殖；转录组测序；差异表达基因；生物膜

英文摘要： Antagonistic Bacillus has been used as a powerful weapon for the suppression of plant pathogenic organisms and the enhancement of plant development in the laboratory and greenhouse experiments. Unfortunately, however, results in the field have been less consistent because of the less competence of root colonization. The colonization of roots by inoculated bacteria is an important step in the interaction between benifical bacteria and the host plant. In the past, scientist focused on the cloning and function analysis of new genes which involved in the biofilm formation, mobility and chemotaxis after induced by root exudates. These results can't evaluate molecular mechanism of root colonization of Bacillus comprehensively. Bacillus subtilis Bs916 has excellent ability of colonization on tomato roots and antagonistic activity on Ralstonia solanacearum in our lab.This program aimed to evaluate the molecular mechanism of root colonization and expansion of Bacillus. According to the colonization laws of Bs916, the rhizosphere bacterial cells would be collected at different time points and performed RNA sequence for gene expression profile analysis. The different expression genes which relate to colonization will be screened based on the Bs916 genome sequence data. Subsequently, some key genes will be given deep invest

英文关键词： Bacillus；colonization；RNA-seq；Differentially expressed genes；Biofilm