一氧化碳调节昼夜节律及代谢的机制研究

项目名称： 一氧化碳调节昼夜节律及代谢的机制研究

项目编号： No.81460212

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 郑敏

作者单位： 延边大学

项目金额： 47万元

中文摘要： 昼夜节律调控着生物体一系列生理活动，包括生物体的新陈代谢。哺乳动物中，生活环境改变而诱导的节律紊乱或遗传因素引起的生物钟基因改变都会使机体的代谢系统平衡失调。昼夜节律与机体代谢过程的同步稳定是预防心血管疾病及代谢疾病的重要因素。越来越多的研究认为，气体一氧化碳是重要的神经传导物质，一氧化碳通过与时钟基因NPAS2结合调节其DNA结合活性而影响昼夜节律。最近， 我们发现一氧化碳通过调节内质网应激改善小鼠高脂饮食引起的肥胖。 本研究通过基因芯片检测技术（microarray）、实时定量PCR技术检测一氧化碳对小鼠生物钟基因表达节律的改变及代谢的影响。本研究将为一氧化碳作为潜在的能够治疗昼夜节律紊乱及代谢疾病的靶点提供理论及实验依据。

中文关键词： 一氧化碳；昼夜节律；代谢

英文摘要： Circadian rhythms control a wide variety of physiological events, including metabolism, in all organisms. In mammals, both environmentally induced circadian disruption as well as genetic aberrations in circadian clock machinery can lead to metabolic disorders. Synchronizing rhythms of behaviour and metabolic processes is important for cardiovascular health and preventing metabolic diseases. The gas CO are increasingly appreciated as major neurotransmitters. Circadian rhythms and neurotransmission by showing that NPAS2 is a hemoprotein whose DNA binding activity is selectively regulated by CO. We have previously shown that CO reversed high-fat diet-induced-obesity by modulating the ER stress response in mice. In this study, the effects of CO on circadian rhythm and metabolism will be studied in vivo using microarryay, Real-time quantitative RT-PCR analysis,etc. This study will provide a theoretic and experimental basis for understanding CO as potential therapeutic target for circadian rhythm dsorders and metabolic disease.

英文关键词： carbon monoxide;circadian rhythm;metabolism