玉米穗粒腐病表观遗传修饰及关键调控因子的鉴定

项目名称： 玉米穗粒腐病表观遗传修饰及关键调控因子的鉴定

项目编号： No.31471513

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 袁广胜

作者单位： 四川农业大学

项目金额： 84万元

中文摘要： 玉米穗粒腐病是世界范围里广泛发生的一种真菌性病害，特别是在我国高温高湿的西南地区该病害流行甚广，造成了玉米产量的巨大损失和品质的严重下降，国内外学者从生理生化、抗性遗传关系、QTL定位、抗病基因克隆、信号转导、抗病分子机理等方面展开了广泛而深入的研究。随着生物科学的发展，植物表观遗传学的研究已成为当今新的焦点，本项目拟在前期研究的基础上，以玉米抗（Bt-1）、感（掖478）穗粒腐病自交系为研究材料，利用高通量甲基化技术检测自交系在病原胁迫下DNA甲基化的动态变化情况，获得甲基化水平存在差异的基因片段；同时采用组蛋白检测技术解析病原胁迫后组蛋白修饰特征。通过关联分析，确定引起玉米穗粒腐病的关键调控因子，采用TALEN靶向基因敲除技术对关键调控因子进行功能验证，探明其中关键调控分子的功能、结构及相互作用网络，进而为玉米穗粒腐病抗病分子机制的研究奠定基础。

中文关键词： 玉米；穗粒腐病；植物表观遗传学；DNA甲基化

英文摘要： Ear rot is a destructive disease in maize caused by Fusarium verticillioides (FV), which results in reduction of grain yield and increase of risks in raising livestock by mycotoxins production. Especially, a high incidence of ear rot occurs in the moist and humid regions of Southwest China, as well as other regions with similar longitude in other countries. In recent years, considerable progresses have been made in understanding the resistant system involved in maize ear rots infected by FV, including physiological and biochemical characteristics, resistant genetic relationship, QTL, isolation of disease resistance genes, characterization of defense responses, and elucidation of signal transduction leading to activation of defense responses. In addition, plant epigenetics research has become the new focus today according to biology development. Based on previous research, we used two maize cultivars, Bt-1 and Ye478 as completely resistant and highly susceptible to FV due to many years evaluation of field in southwest of China in this study to characterize the specific host response to FV infection. To better know the processes involved of defense in maize ear rot upon the FV infection, the DNA methylation change was investigated through Illumina Methylated DNA Immunoprecipitation Sequencing (MeDIP Sequencing) to screen out special differentially regulated genes. At the same time, the modulation of histone modifications were detected by Chromatin Immunoprecipitation (ChIP). The key transcription factors were screened out based on the association analysis from the data of upper results. The function of the key transcription factors by using TALEN targeted gene knockout technology. This study could provide important information on understanding the defense mechanisms to combat invasion in maize ear rot.

英文关键词： Maize;Ear rot;Plant epigenetics;DNA methylation