重组噬菌体对沙眼衣原体作用及机制的探索

项目名称： 重组噬菌体对沙眼衣原体作用及机制的探索

项目编号： No.31500157

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 邵丽丽

作者单位： 天津医科大学

项目金额： 20万元

中文摘要： 沙眼衣原体泌尿生殖道感染是国内外最常见的性传播疾病，可引起严重并发症。很多病人迁延难治，也没有有效的疫苗。国际上新近认为衣原体噬菌体可能是最有希望的生物治疗手段，但迄今国内外尚未发现沙眼衣原体噬菌体。课题组前期研究（国家自然基金30671879）发现具有宿主识别功能的优化GPIC噬菌体Vp1蛋白可特异结合在沙眼衣原体多形态膜蛋白I（Pmp I）上，产生抑制效应，并在沙眼衣原体感染患者的分泌物标本中检测到衣原体噬菌体Vp1核酸片段。本课题拟在前期基础上用上述两种Vp1片段与不同类别噬菌体重组，应用本实验室前期研制出的抗Vp1单克隆抗体和各噬菌体标记物质，结合荧光、胶体金标记，在免疫电镜和共聚焦显微镜下，观察我们构建的12种噬菌体的透膜能力、在沙眼衣原体各生活周期中的生长繁殖和分布，以及宿主沙眼衣原体被抑制、破坏的关系，探讨噬菌体对沙眼衣原体可能作用机制。

中文关键词： 沙眼衣原体；噬菌体；；致病机制

英文摘要： Chlamydia trachomatis urogenital infection is the most common sexually transmitted disease all over the world in recent years, which has many complications, prolonged refractory and lack of effective vaccines. The latest international thought is that Chlamydia phage is expected to serve as an effective means to solve this problem, but have not yet been found it in C.t.Our research group (National Natural Science Foundation 30671879) discovered that the optimized GPIC phage Vp1 with host recognition function could bind in Chlamydia trachomatis polymorphic membrane protein I (Pmp I) specifically and produced inhibition of Chlamydia trachomatis .And detected Chlamydia phage Vp1 nucleic acid fragment in patient secretion samples of Chlamydia trachomatis infection in our lab. This thesis intends to rebuild new engineered-phages using the Vp1 and the differential phages. And using anti Vp1 monoclonal antibody developed early made in our Lab and the phage labeled material, combined with fluorescence, colloidal gold labeling, immune electron microscopy and confocal microscopy, to observe the 12 phages we construct biomembrane and cellular permeability and the growth, reproduction and distribution in the life cycle of Chlamydia trachomatis, and to find the relationship between phage grow with inhibition, destruction of the host Chlamydia trachomatis, to explore this phage possible action mechanism on Chlamydia trachomatis.

英文关键词： chlamydia trachomatis;phage; pathogenesis