牛肉中残留Zeranol诱导人乳腺癌细胞增殖及激活STAT3信号通路的机制研究

项目名称： 牛肉中残留Zeranol诱导人乳腺癌细胞增殖及激活STAT3信号通路的机制研究

项目编号： No.31201424

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 膳食与营养

项目作者： 钟赛意

作者单位： 广东海洋大学

项目金额： 23万元

中文摘要： 乳腺癌是严重威胁女性生命健康最常见的恶性肿瘤之一。从食品中摄入外源类雌激素化合物可能是导致乳腺癌发病率不断升高的因素之一。Zeranol是一种具有雌激素效应的非固醇类化合物，常用于肉牛促生长，也可由存在于霉菌污染的饲料及其原料中的真菌毒素玉米赤霉烯酮加氢还原形成，所以很容易在牛肉组织中残留，然而，对其安全性还不明确。我们的前期研究发现，Zeranol能诱导人乳腺癌细胞系MCF-7的增殖以及引起正常乳腺细胞发生癌化，但对其作用机理以及牛肉中残留痕量Zeranol是否仍具有类雌激素效应和潜在的致乳腺癌作用还有待研究。本研究拟采用原代培养人乳腺癌单层细胞培养、细胞共培养、组织培养以及动物实验，研究牛肉中残留Zeranol对不同人群来源的乳腺癌细胞的增殖影响，并阐明其激活STAT3信号通路的作用机制。以期为Zeranol的膳食暴露风险评估提供参考，为乳腺癌高危人群的靶向预防提供理论依据。

中文关键词： 乳腺癌；Zeranol；细胞增殖；信号通路；

英文摘要： Breast cancer is one of the most commonly diagnosed malignant tumors and the leading cause of cancer death in women. In recent years, the incidence of breast cancer is still increasing all over the world. Estrogen is one of the promoters of breast cancer, playing an important role during the initiation and development of breast cancer. The synthesized estrogens and/or xenoestrogens found in food products or environment, some of which are hormonally active and mimic estrogen, may have negative effect on human health and even induce mammary gland tumor. Therefore, more and more people pay attention to it. Zeranol is a non-steroidal, synthetic resorcyclic acid lactone compound with potent estrogen-like activity, as an anabolic growth promoter for widely use in the beef industry. Fusarium toxin zearalenone in contaminated cereals and feedstuffs also could be metabolized to Zeranol. Therefore, Zeranol may cause residues in cattle beef. However, the safety of using zeranol as a growth promoter has been debated for many years. Consumption of beef containing residues of zeranol and its metabolites may pose a breast cancer risk. Our previous studies have shown that Zeranol stimulated the proliferation of human breast cancer cell line MCF-7 and transfered normal breast cells to cancer cells in vitro. The current study foc

英文关键词： Breast cancer；Zeranol；Cell pliferation；Signal pathway；