蜜蜂 (Apis mellifera) miRNA 的全基因组挖掘及其在级型分化过程中的表达差异分析

项目名称： 蜜蜂 (Apis mellifera) miRNA 的全基因组挖掘及其在级型分化过程中的表达差异分析

项目编号： No.30871830

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 胡福良

作者单位： 浙江大学

项目金额： 32万元

中文摘要： miRNA是生物体内调控其他功能基因表达的重要调控分子，在生物的生长发育过程中发挥着重要作用。蜜蜂作为重要的经济昆虫和模式生物，其基础研究的深入，将有利于进一步开发其经济价值和理论研究价值。本项目利用高通量测序的实验方法，对蜜蜂基因组中的miRNA 进行较为系统的挖掘，并识别出在蜂王和工蜂分化过程中差异表达的miRNA和mRNA。结果miRNA文库测序获得了36,796,459个原始片段，预测得到267个新的蜜蜂miRNA。此外，构建了4个文库，4日龄蜂王（QL4）和工蜂幼虫(WL4)，以及5日龄蜂王(QL5)和工蜂幼虫(WL5)，分别得到了50,191,699、 57,628,541、56,613,619和58,626,829个原始序列，并从中识别出了4500多个蜂王和工蜂分化过程中差异表达的mRNA。级型分化的miRNA测序已经完成，目前正在进行数据的分析和文章写作。本项目的研究首次大规模地获得较为系统可信的蜜蜂miRNA，筛选级型分化过程中差异表达的miRNA和mRNA，为进一步研究蜜蜂发育过程及社会性行为调控中的基因表达机制奠定基础。

中文关键词： miRNA；mRNA；级型分化；RNA-Seq；蜜蜂

英文摘要： miRNAs are a class of key regulatory molecules that can regulate the expression of other functional genes in multicellular organisms which would then play important regulatory roles in organism development. Honey bee (Apis mellifera L) is both an economically important insect and a model organism. Development of fundamental investigation in honeybee will contribute to further exploitation of its economic and theoretical research value. Here, we systematically exploitation of miRNAs from honeybee genome using the high-throughput sequencing system. In addition, we identified the differentially expressed miRNAs /mRNAs genes during the period of differentiation of queen larvae and worker larvae using the same sequencing method. We obtained a total of 36,796,459 raw sequences, from which we predicted 267 novel honey bee miRNAs representing 380,182 short reads. Besides, four libraries prepared from QL and WL at fourth- (L4) and fifth-instar (L5) generated 50,191,699 (QL4), 57,628,541 (WL4), 56,613,619 (QL5), and 58,626,829 (WL5) usable reads, respectively，among which, more than 4,500 Differentially Expressed Genes (DEGs) between castes were identified. Although the sequencing was finished and now we are analyzing the data and writing the paper, the study of differentially expressed miRNAs between QL and WL is not finished yet. This was the first large-scale acquisition of credible honeybee miRNAs and obtained a preliminary understanding of differential expression of miRNAs/mRNAs during caste differentiation. What is more important is that we set up and perfect an experimental and analysis platform which is suitable for honeybee miRNAs study, as well as to lay a foundation for further study on the gene expression mechanism of honeybee development and social behavior regulation.

英文关键词： miRNA；mRNA；caste differentiation；RNA-Seq；honey bee