基于微电极阵列的高效细胞电融合方法研究

项目名称： 基于微电极阵列的高效细胞电融合方法研究

项目编号： No.30870661

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 郑小林

作者单位： 重庆大学

项目金额： 36万元

中文摘要： 细胞融合方法是细胞工程研究的一个重要工具。它在遗传学、杂交育种、发育生物学、医药、食品及农业等方面有广泛的应用价值。尽管传统电融合方法已经广泛应用，但仍是一种随机融合的方法，融合方向不可控制，融合率较高但目标配型的融合成功率仍很低。利用微系统和微操作的细胞电融合技术虽然得到了很大的发展，但是还难如人意。 本项目在前期研究基础上,拟研究一种利用微流控芯片分析和低压电融合技术来实现高效细胞电融合的方法。该方法在现有的芯片上细胞电操作研究的基础上，结合了层流分离、介电电泳分离、荧光标记分离、细胞介电电泳和电脉冲穿孔手段，可以在一个集成的微流控芯片平台上进行细胞样品预处理、细胞的精确控制、细胞的高效融合和融合细胞分离等一系列过程，达到高效的细胞融合和自动筛选。该方法可望克服传统细胞融合仪器价格贵、细胞可控性差、融合效率低，融合细胞难以分离的缺点。对现代医学，农业等相关领域的研究工作有重要意义。

中文关键词： 微流控；微全分析；细胞电融合；高通量

英文摘要： Cell fusion method is a very important tool in cell engineering research.It have wide application in immunological, crossbreeding, developmental biology, medical, food and agriculture. Although traditional cell fusion has wide application, it is a random method and not controllable. It has high fusion efficiency and low success ratio of desired pairing. Microsystem and micromanipulation based cell electrofusion technology has achieved rapid development, but it cannot satisfy practical requirement. Based on the previous research, a high-efficiency cell electrofuison method based on microfluidic chip analysis and low-voltage electrofusion technology is proposed. It ultilizes existing on-chip cell electric manipulation and combined with laminar separation, dielectrophoretic separation, fluorescent lable based separation, cell dielectrophoresis, cell electroporation, to realize cell sample preparation, precise control, high-efficiency fusion and sorting of fused cells on an integrated chip, and achieve high-efficiency fusion and sorting. This method can overcome the defects of traditional method such as expensive, low controllability, low fusion efficiency, difficult for separation. It is of important significancy in modernm medicine and agriculture.

英文关键词： microfluidic; microTAS; cell fusion; high-throughput