中空多孔SiO2纳米微球的自刻蚀制备及其亲和分离蛋白质研究

项目名称： 中空多孔SiO2纳米微球的自刻蚀制备及其亲和分离蛋白质研究

项目编号： No.21271062

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 赵彦保

作者单位： 河南大学

项目金额： 85万元

中文摘要： 亲和沉淀是纯化蛋白质、酶等生物大分子最有效的方法之一。针对当前无机微/纳米微粒亲和载体比表面小、配体结合量有限和吸附容量低等缺点，本项目拟利用中空多孔SiO2纳米微球为亲和沉淀的载体，以提高其对目标蛋白的亲和分离效率。通过研究不同结构SiO2纳米微球的自刻蚀行为，总结自刻蚀理论与实验技术，实现中空多孔SiO2纳米微球结构与性能的有效调控。 在中空多孔SiO2纳米微球表面接枝巯基、氨基、羧基等基团或利用硅烷偶联剂水解制备功能化中空多孔微球，研究表面活性基团的取向与分布规律，调控表面活性基团的种类和浓度；通过引入间臂分子，调节配体结合方式及其空间结构，实现不同亲和配体的有效结合或在微球表面络合上金属离子；研究中空多孔SiO2纳米微球对目标蛋白的亲和吸附行为；建立微球结构、表面特性、配体结合方式与吸附容量的构效关系，优化结构，为中空多孔SiO2纳米微球用作亲和沉淀的载体提供基础数据和理论依据。

中文关键词： 二氧化硅；中空纳米微球；蛋白质纯化；自刻蚀；

英文摘要： Affinity precipitation is one of the most effective methods of purifying proteins, enzymes and other biomolecules. In view of the problem of small ratio surface, limited amount of binding ligand and low adsorption capacity existed in the affinity support of inorganic micro/nano particles, this project using hollow porous SiO2 nanospheres (HPSNSs) as a novel support for affinity precipitation is proposed to improve the separating efficiency of target proteins. It aims to study systematically how SiO2 nanospheres with different structures are converted via hydrothermal treatment into hollow structure, improve the self-etching theory and experimental technique, and tailor the structure and property of HPSNSs. It is also intended to activate the obtained HPSNSs by grafting thiol, amino and carboxyl groups or hydrolyze directly silane coupling agents to produce HPSNSs with active groups, study the orientation and distribution of active groups on the surface of HPSNSs, and tune the kind and concentration of active groups. It would utilize spacer molecules to tune the binding mode and spatial structure of affinity ligands and bond chemically affinity ligands or immobilize metal ions on the surface of HPSNSs. It would study fully the specific adsorption behavior of target proteins on the HPSNSs, relate their structure,

英文关键词： SiO2；hollow nanosphere；protein purification；self etching method；