机体锌稳衡在锌抗肉鸡沙门氏菌感染的作用及机理研究

项目名称： 机体锌稳衡在锌抗肉鸡沙门氏菌感染的作用及机理研究

项目编号： No.31472118

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 张克英

作者单位： 四川农业大学

项目金额： 85万元

中文摘要： 沙门氏菌感染不仅导致肉鸡肠道炎症、生产性能下降，而且可能污染禽肉引发人食入性疾病,预防和控制家禽沙门氏菌感染非常重要。锌是生物必需的微量元素，具有重要的生物学功能。哺乳动物主要通过锌转移蛋白ZNT(Slc30a)与ZIP（Slc39a）家族调控锌的吸收与转运来维持机体的锌稳衡，保证其正常的生理功能。在鼠上的研究表明，锌具有“营养免疫作用”。在细菌感染时，机体锌稳衡发生改变（血清锌降低与锌被重分布）以减少细菌获取锌而增殖，其机理可能与锌调控钙卫蛋白分泌而螯合细菌可利用的锌有关。目前，在肉鸡上尚未见研究报道。本研究采用动物模型和原位结扎灌注肠段法，从锌吸收、转运与重分布等方面来研究肉鸡在不同锌状态下沙门氏菌感染对机体锌稳衡状态的影响；同时体外研究沙门氏菌感染时肉鸡肠上皮细胞与中性粒细胞的锌稳衡调控，并探讨钙卫蛋白与机体锌稳衡的关系，深入阐述机体锌稳衡在锌抗肉鸡沙门氏菌感染的作用及机理。

中文关键词： 肉鸡；锌稳衡；沙门氏菌；基因表达

英文摘要： Salmonella enterica serovar Typhimurium (S.Typhimurium) is a gut pathogen that causes an acute gastroenteritis characterized by inflammatory diarrhea. Food-borne diseases in humans caused by members of Salmonella remain a major public health concern worldwide and are commonly associated with the consumption of broiler chicken meat contaminated with salmonellas. It is very important to prevent the intestinal infection of chickens with salmonellas. Zinc (Zn) is an essential trace mineral with lots of important function. Zinc homeostasis in body and cell are well controlled by Zn transporter proteins (ZnT 1-10, ZIP 1-15), metallothionein (MT).Research in rat showed that Zn has nutritional immunity with the decrease of free Zn level in serum and Neutrophils to prevent the S.Typhimurium to get the necessary Zn. This was induced by Calprotectin with the secretion stimulated by Zn. There is lack of research papers about the role of Zn-homeostasis in broiler with S.Typhimurium infection. In this study, we study the Zn-homeostasis in broiler body, tissue, cells under S.Typhimurium infection by using feeding trial, the method of in situ ligated Loops in vivo, and cell culturation in vitro, while the relationship of Zn-homeostasis with the expression of Zn transporter proteins (ZnT 1-10, ZIP 1-15), metallothionein (MT) and Calprotectin mPNA．The purpose is to understand the role of Zn-homeostasis in body, tissues, and cells in Zn anti-Salmonella infection.

英文关键词： broiler;Zn homeostasis;salmonella;gene expression