不同证型股骨头坏死与MRI、病理及分子分型相互关系的探讨研究

项目名称： 不同证型股骨头坏死与MRI、病理及分子分型相互关系的探讨研究

项目编号： No.81473697

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 何伟

作者单位： 广州中医药大学

项目金额： 73万元

中文摘要： 股骨头坏死（ONFH）是一类在基因、病理和生物学行为方面具有高度异质性的疾病，与成骨细胞和脂肪细胞以及破骨细胞之间复杂的联系密切相关。分子生物学因素决定ONFH的生物学行为和组织病理学改变，进而决定ONFH的影像学表现。但是，对调控成骨细胞、破骨细胞和脂肪细胞的重要基因是否影响ONFH在发病人群中的高度异质性尚无明确报道。本课题①拟对较大样本ONFH病例（至少300例）的MRI各成像切面表现与手术切除股骨头标本对应层面的病理组织学特点进行对比，探讨不同组织学类型ONFH的MRI表现特征及其相应的病理组织学基础；②运用免疫组化和荧光原位杂交的方法，对标本进行成骨细胞分化、破骨细胞分化、脂肪细胞分化相关蛋白和基因检测，对ONFH进行分子分型，分析ONFH的MRI表现与其分子分型的相关性；③分析ONFH的中医证型与分子分型的相关性，为ONFH中医辨证治疗和判断预后提供依据。

中文关键词： 股骨头坏死；中医证型；磁共振；病理；分子分型

英文摘要： Osteonecrosis (ONFH) is a class of diseases with a high degree of heterogeneity in the gene , pathological and biological behavior is closely related to the complex links between bone and fat cells and osteoclasts. ONFH molecular biological factors determine the biological behavior and pathological changes , and then decide ONFH of imaging . However , the regulation of osteoblasts, osteoclasts and important genes are affected ONFH fat cells in the pathogenesis of highly heterogeneous population no clear reports. This paper intends to larger sample ① ONFH cases ( at least 300 cases ) of MRI imaging section of each performance and surgical resection specimens corresponding to the level of the femoral head histopathological features comparing MRI features to explore different histological types and their corresponding ONFH histopathological basis; ② using immunohistochemistry and fluorescence in situ hybridization method, the specimens osteoblast differentiation, osteoclast differentiation, adipocyte differentiation-related protein and genetic testing for ONFH molecular typing analysis ONFH MRI findings and their molecular typing correlation; ③ ONFH analysis of TCM and molecular typing of relevance for ONFH TCM treatment and prognosis provide evidence .

英文关键词： Osteonecrosis of the femoral head;syndrome type of TCM;MRI;histopathology;molecular subtype