用于肿瘤标志物检测的稀土掺杂超小MF2(M=Ca,Sr,Ba)纳米荧光探针及其发光物理

项目名称： 用于肿瘤标志物检测的稀土掺杂超小MF2(M=Ca,Sr,Ba)纳米荧光探针及其发光物理

项目编号： No.11304314

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 郑伟

作者单位： 中国科学院福建物质结构研究所

项目金额： 30万元

中文摘要： 稀土掺杂超小(<10 nm) MF2（M = Ca, Sr, Ba）纳米晶因其良好的生物相容性，有望成为一类高效的荧光探针应用于生物医学领域，但目前受到了荧光量子产率低的限制。本项目致力于研发具有高效发光的稀土掺杂超小MF2纳米荧光探针，开展超小MF2纳米晶及其核壳结构的可控合成、表面修饰和发光物理研究，探索稀土离子异价掺杂体系结构和发光性能的关系，揭示纳米晶的尺寸和表面结构对发光强度的影响，寻找提高小尺寸纳米晶发光效率的途径，从中筛选出至少一种高效、实用的纳米荧光探针，并实现其对人体血清中肿瘤标志物如尿激酶受体（uPAR）的高灵敏特异性体外检测以及肿瘤靶向生物成像。预期本项目研制的超小纳米荧光探针在荧光强度、光稳定性、生物安全性和灵敏度等方面都表现出优于目前商用的分子探针如有机荧光染料和稀土螯合物的良好性能，在生物科学和临床检测等领域将发挥重要的作用。

中文关键词： CaF2；稀土掺杂；纳米荧光探针；肿瘤标志物；生物检测

英文摘要： Ultrasmall (<10 nm) MF2 (M = Ca, Sr, Ba) nanocrystals (NCs) doped with trivalent lanthanide ions (Ln3+), owing to their excellent bicompatibility, could serve as excellent fluorescencent probes for biomedical applications. However, currently they are still limited in practical bio-applications due to the low photoluminescent quantum yield. In this project, we aim to synthesize ultrasmall Ln3+-doped MF2 nanoprobes that are highly emissive. The synthesis, surface modification, and photophysics of these ultrasmall MF2:Ln3+ NCs and their core/shell counterparts will be systematically studied. The relationship between the structure and the photoluminescence (PL) properties in the matrices heterovalently doped with Ln3+ will be investigated; the influence of the particle size and surface structure on the PL intensity will be revealed, in an effort to improve the luminescence efficiency. To this regard, we'll develop at least one efficient and pratical nanoprobe used for sensitive and specific detection of tumor markers in human serum such as urokinase-type plasminogen activator receptor (uPAR), and for targeted tumor imaging.Such ultrasmall MF2:Ln3+ nanoprobes are expected to show much more superior features in the PL intensity, photostability, biosafety and sensitivity than commercial molecular probes such as organ

英文关键词： CaF2；lanthanide-doped；luminescent nanoprobes；tumor marker；biodetection