细胞凋亡在假禾谷镰刀菌侵染过程中的功能分析

项目名称： 细胞凋亡在假禾谷镰刀菌侵染过程中的功能分析

项目编号： No.31501594

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 陈琳琳

作者单位： 河南农业大学

项目金额： 20万元

中文摘要： 细胞凋亡调控多种病原真菌的生长、发育及致病过程，但在镰刀菌中的发生和调控机制尚不清楚。我们前期利用TUNEL法检测到过氧化氢可以诱导假禾谷镰刀菌分生孢子细胞凋亡的发生，并发现多个细胞凋亡相关基因可能参与调控病原菌的侵染过程。本研究拟通过检测细胞凋亡在假禾谷镰刀菌生长、发育及侵染等阶段的发生规律，明确其细胞凋亡的发生特点；通过对细胞凋亡信号通路上关键基因的功能分析，揭示细胞凋亡对假禾谷镰刀菌侵染过程的调控机制；鉴定细胞凋亡蛋白的互作蛋白，解析其信号通路。研究结果为进一步深入探索假禾谷镰刀菌的致病机制以及以调控细胞凋亡为途径的病害防治提供理论依据。

中文关键词： 假禾谷镰刀菌；细胞凋亡；功能基因；致病性

英文摘要： Apoptosis is known to regulate the growth, development and pathogenicity of various pathogenic fungi, but the occurrence and regulation mechanism are still not clear in Fusarium. In previous studies, we found that the number of TUNEL-positive cells was increased after hydrogen peroxide treatment of Fusarium pseudograminearum conidia, which indicated apoptosis occurrence of F. pseudograminearum. Expression levels of fourteen apoptosis-related genes were analyzed, and several genes might be involved in pathogen infection process. Here, apoptosis regularity will be investigated by analyses of F. pseudograminearum TUNEL positive cells at different stages, and the regulation mechanism of F. pseudograminearum infection processes by apoptosis will be elucidated by functional analysis of apoptosis-related genes. The signal pathway of apoptosis in F. pseudograminearum will be analyzed through interactional proteins identification. These results will aid the understanding of apoptosis regulation mechanisms of F. pseudograminearum pathogenicity, and provide scientific basis for development of novel control strategies against F. pseudograminearum by regulating cell apoptosis.

英文关键词： Fusarium pseudograminearum;Apoptosis;Functional gene;Pathogenicity