枸杞多糖经TLR/NF-κB通路对2型糖尿病炎症因子生成的抑制

项目名称： 枸杞多糖经TLR/NF-κB通路对2型糖尿病炎症因子生成的抑制

项目编号： No.81460494

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 蔡慧珍

作者单位： 宁夏医科大学

项目金额： 45万元

中文摘要： 近年的研究认为2型糖尿病（T2DM）可能是细胞因子介导的炎症反应，炎症在T2DM的发病中起媒介作用。干预炎症过程同时具有减轻胰岛素抵抗，减少胰岛β细胞凋亡的作用。枸杞多糖是我国传统名贵中药材--枸杞的主要活性成分。在前期的研究中我们发现枸杞多糖能够抑制T2DM患者血清炎症因子TNF-α的水平，进而缓解T2DM患者的胰岛素抵抗，但其机制尚不清楚。TLRs是一类能够识别潜在病原体结构的配体。其中TLR2/4已被证实在T2DM的发展中占有重要作用。TLR2/4能识别多糖，并通过TLR/NF-κB信号传导通路参与多糖对免疫系统的调节。由此我们猜测枸杞多糖是否通过TLR/NF-κB信传导号通路对炎症因子产生了抑制，并进而缓解了T2DM的胰岛素抵抗。因此，本文拟从TLR/NF-κB信号传导通路入手，研究枸杞多糖抑制T2DM炎症因子产生的机制，以期为枸杞多糖应用于T2DM治疗提供理论基础。

中文关键词： 2型糖尿病；营养干预；分子机制

英文摘要： Recent studies suggest that type 2 diabetes (T2DM) may be an inflammatory processes mediated by cytokines. Inflammation acts as an intermediary in the pathogenesis of T2DM. Intervention of inflammatory processes may reduce insulin resistance as well as islet beta cell apoptosis. Lycium barbarum polysaccharide is the main active constituent of famous traditional Chinese medicinal herb - Chinese wolfberry. In previous study, we found that Lycium barbarum polysaccharide could inhibit the serum TNF-α level of T2DM and thereby alleviating insulin resistance in T2DM patients. But its mechanism was not clear. TLRs is a kind of ligand to recognize potential pathogens. TLR2/4 has been shown to play an important role in the development of T2DM. TLR2/4 can identify polysaccharides and is involved in the regulation of immune system through the TLR/NF-κB signal transduction pathway for polysaccharides. Thus, it is hypothesized that TLR/NF-κB signal transduction pathway is inhibited to relieve insulin resistance by Lycium barbarum polysaccharide in T2DM. Therefore, this article studies the inhibited mechanism of inflammatory cytokines by Lycium barbarum polysaccharide through TLR/NF-κB signal transduction pathway in T2DM, in ordor to provide a theoretical basis for the application of Lycium barbarum polysaccharide on T2DM treatment.

英文关键词： type 2 diabetes;Nutrition intervention;Molecular mechanism